Cell Biochemistry and Biophysics ( IF 2.6 ) Pub Date : 2021-06-14 , DOI: 10.1007/s12013-021-01011-x Jun Zhang 1 , Chao Chen 1 , Fengpeng Li 1 , Guangren Gao 1 , Jinguo Fu 1 , Heping Niu 1 , Kai Yu 1 , Lei Wang 1 , Run Guo 1 , Yanfang Wan 1 , Zhanfeng Ma 1 , Qianyu Zhang 1 , Liping Mu 1 , Jing Liu 1 , Jing Yang 1 , Linlin Wang 1
NOS–NO system activity is closely correlated with ischemia–reperfusion injury (IRI) and NOS subtypes were suggested to play different roles in IRI. In this work, the activity of serum NOS, NO levels, and ischemic necrosis after reperfusion in rabbit with AMI at different time was studied. We also explored the NOS–NO system activity changes and its correlation with myocardial ischemia and necrosis. It shows that after reperfusion in rabbits with AMI, NO–NOS system activities present different changes at each time point due to inactivation of NO and iNOS activation, and different experimental animals, ischemia–reperfusion degree, and length of time will also lead to different research results. Therefore, it is necessary to conduct dynamic observation on animals from different species at multi-temporal point under the state of NOS–NO system activities, and simultaneously detect inflammatory factor, MDA, and SOD indexes. Therefore, it is a must to conduct relevant drug research studies to make NOS–NO system activities maintain the level in favor of ideal myocardial ischemia reperfusion.
中文翻译:
AMI兔缺血再灌注后三种NOS-NO系统活性的不同变化模式
NOS-NO系统活性与缺血再灌注损伤(IRI)密切相关,NOS亚型被认为在IRI中发挥不同的作用。本工作研究了AMI兔不同时间再灌注后血清NOS活性、NO水平及缺血坏死情况。我们还探讨了 NOS-NO 系统活性变化及其与心肌缺血和坏死的相关性。说明 AMI 兔再灌注后,由于 NO 失活和 iNOS 激活,NO-NOS 系统活性在各时间点呈现不同的变化,不同的实验动物、缺血再灌注程度、时间长短也会导致不同的研究成果。所以,需要对不同物种的动物在NOS-NO系统活性状态下多时点进行动态观察,同时检测炎症因子、MDA、SOD指标。因此,必须进行相关药物研究,使NOS-NO系统活性维持在有利于理想心肌缺血再灌注的水平。