Black rot of crucifers is one of the most important diseases of wild rocket (Diplotaxis tenuifolia L. (D.C.)) caused by the seedborne pathogen Xanthomonas campestris pv. campestris. From 2005, it frequently affected this cultivation in the south of Italy, leading to heavy crop losses. In the present work, we aimed to describe the physiological and molecular characteristics of twenty X. campestris pv. campestris strains isolated from plants and seeds. Ten Xanthomonas spp. strains contaminating seeds were identified on the basis of molecular characterization and in vivo pathogenicity on a discriminating host range. Some of seed-borne isolates were ascribed to the species Xanthomonas campestris pv. raphani and X. campestris pv. incanae, indicating the occurrence of non-host pathogenic Xanthomonas on wild rocket seeds. As well as the presence of pathogenic bacteria, even non-pathogenic Xanthomonas spp. strains were detected on the seeds, underlying the importance of identifying them to evaluate the suitability of lots intended for sowing. A phylogeny using 69 Gyrase B (gyrB) sequences retrieved from the literature, was also carried out, highlighting species relatedness. Overall, this study provides a comprehensive framework for Xanthomonas species affecting wild rocket in Southern Italy.

十字花科植物黑腐病是由种传病原野油菜黄单胞菌 (Xanthomonas campestris pv. ) 引起的野生火箭 ( Diplotaxis tenuifolia L. (DC))最重要的病害之一。油菜。从 2005 年开始，它经常影响意大利南部的这种种植，导致农作物严重损失。在目前的工作中，我们旨在描述 20 X. campestris pv.的生理和分子特征。从植物和种子中分离出的油菜菌株。十个黄单胞菌属。污染种子的菌株是在分子特征和体内致病性的基础上确定的。一些种子传播的分离株归因于该物种Xanthomonas campestris pv. raphani和X. campestris pv。incanae，表明野生火箭种子上发生了非寄主致病性黄单胞菌。以及致病菌的存在，甚至非致病性黄单胞菌属。在种子上检测到菌株，这是识别它们以评估打算播种的批次的适用性的重要性的基础。还进行了使用从文献中检索到的69 个Gyrase B ( gyrB ) 序列的系统发育，突出了物种相关性。总的来说，这项研究为黄单胞菌提供了一个综合框架 影响意大利南部野生火箭的物种。