Blue light is an important signal that regulates the flowering of strawberry plants. To reveal the mechanism of early flowering under blue light treatment at the transcriptional regulation level, seedlings of cultivated strawberry (Fragaria × ananassa Duch.) “Benihoppe” were subjected to a white light treatment (WL) and blue light treatment (BL) until their flowering. To detect the expression patterns of genes in response to BL, a transcriptome analysis was performed based on RNA-Seq. The results identified a total of 6875 differentially expressed genes (DEGs) that responded to BL, consisting of 3138 (45.64%) downregulated ones and 3737 (54.36%) upregulated ones. These DEGs were significantly enriched into 98 GO terms and 71 KEGG pathways based on gene function annotation. Among the DEGs, the expression levels of genes that might participate in light signaling (PhyB, PIFs, and HY5) and circadian rhythm (FKF1, CCA1, LHY, and CO) in plants were altered under BL. The BBX transcription factors which responded to BL were also identified. The result showed that the FaBBX29, one of strawberry’s BBX family genes, may play an important role in flowering regulation. Our results provide a timely, comprehensive view and a reliable reference data resource for further study of flowering regulation under different light qualities.

中文翻译：

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草莓叶转录组谱分析揭示蓝光处理下的开花调控

蓝光是调节草莓植株开花的重要信号。从转录调控水平揭示蓝光处理早花机制，栽培草莓（Fragaria × ananassaDuch.) “Benihoppe”经过白光处理 (WL) 和蓝光处理 (BL) 直至开花。为了检测响应 BL 的基因表达模式，基于 RNA-Seq 进行转录组分析。结果共鉴定出 6875 个对 BL 有反应的差异表达基因 (DEG)，其中包括 3138 个（45.64%）下调基因和 3737 个（54.36%）上调基因。这些DEG基于基因功能注释被显着富集为98个GO术语和71个KEGG通路。在 DEG 中，可能参与光信号（PhyB、PIF和HY5）和昼夜节律（FKF1、CCA1、LHY和CO ) 在植物中被 BL 改变。还鉴定了响应 BL 的 BBX 转录因子。结果表明，草莓BBX家族基因之一的FaBBX29可能在开花调控中起重要作用。我们的研究结果为进一步研究不同光质量下的开花调控提供了及时、全面的观点和可靠的参考数据资源。