ABSTRACT

The present study reports the fabrication and characterization of nanofibrous scaffold comprising silk fibroin (SF) and polycaprolactone (PCL) incorporated with vitamin D3 (VD3). The optimized SF/PCL/VD3 nanofibers of average diameter 129±19 nm were obtained by electrospinning technique. The results of the contact angle measurements suggested that the presence of VD3 improves the wettability of the fibers and the water contact angle of the PCL nanofibers decreased from 75±1° to 53±1° after SF and VD3 addition. The elastic modulus for PCL nanofiber was increased from 0.78±0.27 MPa to 2.3±1.16 MPa upon the incorporation of SF and VD3. The cumulative release percentage of VD3 from the scaffolds was 48% during 24 hours. According to MTT assay and cell culture, the nanofibrous scaffolds (SF/PCL/VD3) were superior to PCL and SF/PCL scaffolds in improving the attachment and spreading of adipose derived stem cells (ADSCs), therefore, may be potential candidates for bone tissue engineering applications.

摘要

本研究报告了包含丝素蛋白 (SF) 和聚己内酯 (PCL) 与维生素 D3 (VD3) 的纳米纤维支架的制造和表征。采用静电纺丝技术得到优化后的平均直径为129±19 nm的SF/PCL/VD3纳米纤维。接触角测量结果表明，VD3 的存在提高了纤维的润湿性，并且在添加 SF 和 VD3 后，PCL 纳米纤维的水接触角从 75±1° 降低到 53±1°。加入 SF 和 VD3 后，PCL 纳米纤维的弹性模量从 0.78±0.27 MPa 增加到 2.3±1.16 MPa。在 24 小时内，支架中 VD3 的累积释放百分比为 48%。根据 MTT 测定和细胞培养，