ABSTRACT The aim of this study was to evaluate the influence of biodynamic (BD) preparations 500 and 501 on the quality of white mulberry (Morus alba L.) leaves. Leaves of the two mulberry cultivars, Plodovaja 3 and Turchanka, were collected in September 2015 and 2016. Quantitative determinations of phenolic, flavonoid and chlorogenic acid concentrations in mulberry leaves were conducted by UPLC methods. The results showed that the total phenolic compounds concentration (TPCC) and total flavonoid concentration (TFC) in the samples ranged from 4483 to 5005 and from 2944 to 3292 mg ChAE 100 g−1 DM, respectively, with leaves of Turchanka containing the highest concentrations and significant differences between the cultivars for treatments with BD application. The major flavonoids in the mulberry leaves were quercetin-acetylhexoside, isoquercitrin, rutin, kaempferol-acetylhexoside and astragalin, and the concentration of these varied depending on the cultivar (apart from for isoquercitrin and astragalin). The results demonstrated that BD preparations influenced the TPCCs of the two cultivars differently. The application of BD preparation 500 significantly increased the total phenolic and flavonoid concentrations in the leaves of Turchanka by 3.62% and 6.1%, respectively. Using the two preparations together (500 + 501) had significant effects on quercetin-acetylhexoside and kaempferol-acetylhexoside accumulation in the mulberry leaves of both cultivars. Application of preparation 501 significantly reduced the TPCC, TFC and chlorogenic acid concentration in the leaves of Plodovaja 3, compared with the control. The results showed that BD preparations had the greatest positive effect on the accumulation of phenolic compounds in the leaves of the Turchanka cultivar.

中文翻译：

---

生物动力制剂对两个白桑树品种叶片酚类化合物浓度的影响

摘要 本研究的目的是评估生物动力 (BD) 制剂 500 和 501 对白桑 (Morus alba L.) 叶质量的影响。2015 年 9 月和 2016 年收集了两个桑树品种 Plodovaja 3 和 Turchanka 的叶子。通过 UPLC 方法进行了桑叶中酚类、黄酮类和绿原酸浓度的定量测定。结果表明，样品中总酚类化合物浓度 (TPCC) 和总黄酮类化合物浓度 (TFC) 的范围分别为 4483 至 5005 和 2944 至 3292 mg ChAE 100 g-1 DM，其中图尔恰卡叶的浓度最高BD应用处理的栽培品种之间存在显着差异。桑叶中的主要黄酮类化合物是槲皮素-乙酰己糖苷、异槲皮苷、芦丁、山奈酚-乙酰己糖苷和黄芪甲苷，它们的浓度因品种而异（异槲皮苷和黄芪甲苷除外）。结果表明，BD制剂对两个品种的TPCC的影响不同。BD 制剂 500 的施用显着增加了 Turchanka 叶片中总酚类和类黄酮的浓度，分别为 3.62% 和 6.1%。两种制剂一起使用 (500 + 501) 对两个品种桑叶中槲皮素-乙酰己糖苷和山奈酚-乙酰己糖苷的积累有显着影响。与对照相比，501 制剂的施用显着降低了 Plodovaja 3 叶片中的 TPCC、TFC 和绿原酸浓度。