Abstract Balfourodendron riedelianum is a tropical tree endemic to the Atlantic Forest biome that is currently endangered due to forest fragmentation and extensive exploitation. Three populations of the species are conserved ex situ in a provenance and progeny test at the Luiz Antônio Experimental Station, São Paulo State, Brazil. To verify if seven microsatellite loci developed for the species can be used as genetic markers in analyses focused on conservation and sustainable use strategies, leaf tissue samples were collected from the three provenances and from 17 seed trees in one provenance. We analyzed Mendelian inheritance and genetic linkage for the 17 seed trees and genotypic linkage disequilibrium for individuals from the three provenances. For six of the seven loci analyzed, all 17 seed trees showed heterozygosity. The inheritance and genetic linkage analyses were performed using respective locus, while the genotypic linkage disequilibrium analysis was performed for the seven loci. After Bonferroni correction, none of the 75 tests showed deviation from Mendelian segregation and genetic linkage, nor did we detect genotypic linkage disequilibrium. The results suggest that six of the seven loci can be used for population genetics studies on B. riedelianum.

中文翻译：

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通过对孟德尔遗传、遗传连锁和基因型连锁不平衡的分析来验证 Balfourodendron riedelianum 的微卫星位点

摘要 Balfourodendron riedelianum 是大西洋森林生物群落的特有热带树木，目前由于森林破碎化和广泛开发而濒临灭绝。该物种的三个种群在巴西圣保罗州的 Luiz Antônio 实验站进行的种源和后代测试中被移地保护。为了验证为该物种开发的七个微卫星位点是否可以用作侧重于保护和可持续利用策略的分析中的遗传标记，从三个来源和一个来源的 17 棵种子树中收集了叶组织样本。我们分析了 17 棵种子树的孟德尔遗传和遗传连锁以及来自三个来源的个体的基因型连锁不平衡。对于分析的七个基因座中的六个，所有 17 棵种子树都显示杂合性。使用各自的基因座进行遗传和遗传连锁分析，而对七个基因座进行基因型连锁不平衡分析。Bonferroni 校正后，75 项测试中没有一项显示偏离孟德尔分离和遗传连锁，我们也没有检测到基因型连锁不平衡。结果表明，七个基因座中有六个可用于 B. riedelianum 的群体遗传学研究。