This paper aims to propose a new microfluidic portable experimental platform for quick detection of heavy metal ions (HMIs) in picomolar range. The experimental setup uses a microfabricated piezoresistive sensor (MPS) array of eight cantilevers with ion-selective self-assembled monolayer's (SAM).,Most of the components used in this experimental setup are battery operated and, hence, portable to perform the on-field experiments. HMIs (antigen) and thiol-based SAM (antibody) interaction start bending the microcantilever. This results in a change of resistance, which is directly proportional to the surface stress produced due to the mass of targeted HMIs. The authors have used Cysteamine and 4-Mercaptobenzoic acid as a thiol for creating SAM to test the sensitivity and identify the suitable thiol. Some of the cantilevers are blocked using acetyl chloride to use as a reference for error detection.,The portable experimental platform achieves very small detection time of 10-25 min with a lower limit of detection (LOD) 0.762 ng (6.05 pM) for SAM of Cysteamine and 4-Mercaptobenzoic acid to detect Mn2+ ions. This technique has excellent potential and capability to selectively detect Hg2+ ions as low as 2.43 pM/mL using SAM of Homocysteine (Hcys)-Pyridinedicarboxylic acid (PDCA).,As microcantilever is very thin and fragile, it is challenging to apply a surface coating to have selective detection using Nanadispenser. Some of the cantilevers get broken during this process.,The excessive use and commercialization of NPs are quickly expanding their toxic impact on health and the environment. Also, LOD is limited to nanomolar range. The proposed method used the combination of thin-film, NPs, and MEMS-based technology to overcome the limitation of NPs-based technique and have picomolar range of HMIs detection.

中文翻译：

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基于压阻BioMEMS传感器检测重金属离子的微流控实验装置设计

本文旨在提出一种新的微流体便携式实验平台，用于在皮摩尔范围内快速检测重金属离子（HMI）。实验装置使用具有离子选择性自组装单层 (SAM) 的八个悬臂的微制造压阻传感器 (MPS) 阵列。本实验装置中使用的大多数组件都是电池供电的，因此可携带进行田间试验。HMI（抗原）和基于硫醇的 SAM（抗体）相互作用开始弯曲微悬臂梁。这会导致电阻的变化，这与由于目标 HMI 的质量而产生的表面应力成正比。作者使用半胱胺和 4-巯基苯甲酸作为硫醇来创建 SAM，以测试灵敏度并确定合适的硫醇。部分悬臂使用乙酰氯封闭，作为错误检测的参考。便携式实验平台实现了10-25分钟的极短检测时间，SAM的检测下限（LOD）为0.762 ng（6.05 pM）半胱胺和 4-巯基苯甲酸检测 Mn2+ 离子。该技术具有使用同型半胱氨酸 (Hcys)-吡啶二羧酸 (PDCA) 的 SAM 选择性检测低至 2.43 pM/mL 的 Hg2+ 离子的出色潜力和能力。由于微悬臂梁非常薄且易碎，因此应用表面涂层具有挑战性使用 Nanodispenser 进行选择性检测。在此过程中，一些悬臂会断裂。NPs 的过度使用和商业化正在迅速扩大其对健康和环境的毒性影响。此外，LOD 仅限于纳摩尔范围。