Abstract

A method for the quantification of a mixture of fluorescent antibiotics with broad, highly overlapped excitation and emission spectra is proposed. The method enables the simultaneous determination of sarafloxacin, flumequine and ofloxacin by using the fluorescence excitation-emission matrix and performing two different scans, namely, a linear variable angle synchronous fluorimetry scan at 15.6° and an emission scan at λ_exc of 320 nm. This ensures complete resolution of the mixture without the need for a separation technique. Various factors influencing the fluorescence intensity were assessed in order to maximize the sensitivity and obtain an adequate selectivity. The method was successfully used to determine the analytes in pharmaceuticals and rabbit plasma. The results obtained compared favorably with those provided by HPLC.

中文翻译：

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使用全荧光光谱法同时测定兔血浆中氟喹诺酮类的高灵敏度

摘要

提出了一种量化具有广泛、高度重叠的激发和发射光谱的荧光抗生素混合物的方法。该方法通过使用荧光激发-发射矩阵并执行两种不同的扫描，即 15.6° 线性可变角度同步荧光扫描和 λ _exc发射扫描，能够同时测定沙拉沙星、氟甲喹和氧氟沙星。320 纳米。这确保了混合物的完全分离，而无需分离技术。评估影响荧光强度的各种因素，以最大限度地提高灵敏度并获得足够的选择性。该方法已成功用于测定药物和兔血浆中的分析物。所得结果与 HPLC 提供的结果相比具有优势。