ABSTRACT

Introduction

Toxoplasmosis is a globally distributed parasitic infection that can be particularly severe when opportunistic or congenital. Its diagnosis requires accurate and rapid techniques that rely mainly on serology and molecular methods.

Areas covered

The aim of this review was to discuss the positioning of the molecular diagnosis of toxoplasmosis according to the different clinical situations possibly resulting from infection with T. gondii, and to detail recent developments in this technique. The English and French literature were searched with the following keywords: ‘Toxoplasmosis’, “Molecular diagnosis“ and ‘PCR’.

Expert opinion

Molecular techniques have revolutionized the diagnosis of toxoplasmosis, and practices have considerably evolved over the past decades. However, there is still a high degree of inter-laboratory heterogeneity which impairs comparisons between results and studies. Efforts to standardize practices are underway.

中文翻译：

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弓形虫病的分子诊断：最新进展和对未来的展望

摘要

介绍

弓形虫病是一种全球分布的寄生虫感染，当机会性或先天性时可能特别严重。它的诊断需要主要依靠血清学和分子方法的准确和快速的技术。

涵盖的领域

本综述的目的是根据弓形虫感染可能导致的不同临床情况，讨论弓形虫病分子诊断的定位，并详细介绍该技术的最新进展。使用以下关键字搜索英语和法语文献：“弓形虫病”、“分子诊断”和“PCR”。

专家意见

分子技术已经彻底改变了弓形虫病的诊断，并且在过去的几十年中实践有了很大的发展。然而，仍然存在高度的实验室间异质性，这损害了结果和研究之间的比较。正在努力使做法标准化。