The present study investigates the effects of enterocin Ent M and durancin Ent ED26E/7 applied separately and in combination on the intestinal microbiota, caecal enzymatic activity, and fermentaion of rabbits. Eighty rabbits (M91 meatline, aged 5 weeks, both sexes) were divided into groups E (Ent M; 50 µL/animal/day), D (Ent ED26E/7; 50 µL/animal/day), E + D (Ent M + Ent ED26E/7), and control (C). The additives were administered in drinking water for 21 days. Antimicrobial activity of Ent M and Ent ED26E/7 on coliforms (E, E + D: P < 0.001) and pseudomonads (D: P < 0.05) in feces was noted, compared to C. Ent M and Ent ED26E/7 application stimulated caecal enzymatic activity in rabbits. Pectinolytic (E vs. D, E + D: P < 0.01), inulolytic (E vs. E + D: P < 0.01; E vs. C: P < 0.05), and amylolytic (E vs. D, E + D. P < 0.001; E vs. C: P < 0.01) activities were influenced by Ent M, while cellulolytic (D vs. E + D: P < 0.01) and inulolytic (D vs. E + D, C: P < 0.01) activities by Ent ED26E/7 treatment. The cellulolytic and pectinolytic acitivities changed with time. Treatment × time interaction was detected for cellulose and xylan degradation. During Ent M and Ent ED26E/7 treatment, increased ammonia, lactic, butyric and iso-valeric acid, and lower acetic, propionic, iso-butyric, valeric, and caproic acid concentrations were noted. It can be concluded that Ent M and Ent ED26E/7 application can improve rabbit health due to reduced spoilage microbiota and enhanced caecal enzymatic activity.

本研究探讨了单独和联合应用 enterocin Ent M 和 durancin Ent ED26E/7 对兔肠道微生物群、盲肠酶活性和发酵的影响。将 80 只兔子（M91 肉线，5 周龄，雌雄同体）分为 E 组（Ent M；50 µL/动物/天）、D（Ent ED26E/7；50 µL/动物/天）、E + D（Ent M + Ent ED26E/7) 和对照 (C)。添加剂在饮用水中给药 21 天。 与 C 相比，Ent M 和 Ent ED26E/7 对粪便中的大肠菌群 (E, E + D: P  < 0.001) 和假单胞菌 (D: P < 0.05) 具有抗菌活性。Ent M 和 Ent ED26E/7 应用刺激兔盲肠酶活性。Pectinolytic (E vs. D, E + D: P  < 0.01), inulolytic (E vs. E + D:P  < 0.01；E vs. C：P  < 0.05）和淀粉分解（E vs. D，E + D. P  < 0.001；E vs. C：P  < 0.01）活性受 Ent M 影响，而纤维素分解（D vs. E + D: P  < 0.01) 和菊粉分解 (D vs. E + D, C: P < 0.01) Ent ED26E/7 处理的活性。纤维素分解和果胶分解活性随时间变化。检测到纤维素和木聚糖降解的处理×时间相互作用。在 Ent M 和 Ent ED26E/7 处理期间，注意到氨、乳酸、丁酸和异戊酸增加，而乙酸、丙酸、异丁酸、戊酸和己酸浓度降低。可以得出结论，应用 Ent M 和 Ent ED26E/7 由于减少了腐败菌群和增强了盲肠酶活性，可以改善兔子的健康。