当前位置: X-MOL 学术Science › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Analysis of multispectral imaging with the AstroPath platform informs efficacy of PD-1 blockade
Science ( IF 56.9 ) Pub Date : 2021-06-11 , DOI: 10.1126/science.aba2609
Sneha Berry 1, 2, 3 , Nicolas A Giraldo 4 , Benjamin F Green 1, 2, 5 , Tricia R Cottrell 4 , Julie E Stein 4 , Elizabeth L Engle 1, 2, 5 , Haiying Xu 1, 2, 5 , Aleksandra Ogurtsova 1, 2, 5 , Charles Roberts 2, 5 , Daphne Wang 5 , Peter Nguyen 5 , Qingfeng Zhu 4 , Sigfredo Soto-Diaz 2, 5 , Jose Loyola 2, 5 , Inbal B Sander 5 , Pok Fai Wong 6 , Shlomit Jessel 6 , Joshua Doyle 7, 8 , Danielle Signer 5 , Richard Wilton 7, 8 , Jeffrey S Roskes 7, 8 , Margaret Eminizer 7, 8 , Seyoun Park 1, 9 , Joel C Sunshine 5 , Elizabeth M Jaffee 1, 2, 3 , Alexander Baras 4 , Angelo M De Marzo 3, 4 , Suzanne L Topalian 2, 10 , Harriet Kluger 11 , Leslie Cope 1, 2, 12 , Evan J Lipson 1, 2, 3 , Ludmila Danilova 1, 2, 12 , Robert A Anders 1, 2, 4 , David L Rimm 6 , Drew M Pardoll 1, 2, 3 , Alexander S Szalay 1, 7, 8 , Janis M Taube 1, 2, 4, 5
Affiliation  

Next-generation tissue-based biomarkers for immunotherapy will likely include the simultaneous analysis of multiple cell types and their spatial interactions, as well as distinct expression patterns of immunoregulatory molecules. Here, we introduce a comprehensive platform for multispectral imaging and mapping of multiple parameters in tumor tissue sections with high-fidelity single-cell resolution. Image analysis and data handling components were drawn from the field of astronomy. Using this “AstroPath” whole-slide platform and only six markers, we identified key features in pretreatment melanoma specimens that predicted response to anti–programmed cell death-1 (PD-1)–based therapy, including CD163+PD-L1 myeloid cells and CD8+FoxP3+PD-1low/mid T cells. These features were combined to stratify long-term survival after anti–PD-1 blockade. This signature was validated in an independent cohort of patients with melanoma from a different institution.



中文翻译:

使用 AstroPath 平台分析多光谱成像可了解 PD-1 阻断的功效

用于免疫治疗的下一代基于组织的生物标志物可能包括同时分析多种细胞类型及其空间相互作用,以及免疫调节分子的不同表达模式。在这里,我们介绍了一个综合平台,用于在具有高保真单细胞分辨率的肿瘤组织切片中进行多光谱成像和多个参数的映射。图像分析和数据处理组件来自天文学领域。使用这个“AstroPath”全载玻片平台和仅六个标记,我们确定了预处理黑色素瘤标本中的关键特征,这些特征预测了对基于抗程序性细胞死亡 1 (PD-1) 的治疗的反应,包括 CD163 + PD-L1 骨髓细胞和 CD8 + FoxP3 +PD-1低/中T 细胞。结合这些特征对抗 PD-1 阻断后的长期生存进行分层。这一特征在来自不同机构的黑色素瘤患者的独立队列中得到验证。

更新日期:2021-06-11
down
wechat
bug