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Minor pilins are involved in motility and natural competence in the cyanobacterium Synechocystis sp. PCC 6803
Molecular Microbiology ( IF 3.6 ) Pub Date : 2021-06-11 , DOI: 10.1111/mmi.14768
Sabrina Oeser 1 , Thomas Wallner 1 , Nils Schuergers 1 , Lenka Bučinská 2 , Shamphavi Sivabalasarma 3, 4 , Heike Bähre 5 , Sonja-Verena Albers 3, 4 , Annegret Wilde 1
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Cyanobacteria synthesize type IV pili, which are known to be essential for motility, adhesion and natural competence. They consist of long flexible fibers that are primarily composed of the major pilin PilA1 in Synechocystis sp. PCC 6803. In addition, Synechocystis encodes less abundant pilin-like proteins, which are known as minor pilins. In this study, we show that the minor pilin PilA5 is essential for natural transformation but is dispensable for motility and flocculation. In contrast, a set of minor pilins encoded by the pilA9-slr2019 transcriptional unit are necessary for motility but are dispensable for natural transformation. Neither pilA5-pilA6 nor pilA9-slr2019 are essential for pilus assembly as mutant strains showed type IV pili on the cell surface. Three further gene products with similarity to PilX-like minor pilins have a function in flocculation of Synechocystis. The results of our study indicate that different minor pilins facilitate distinct pilus functions. Further, our microarray analysis demonstrated that the transcription levels of the minor pilin genes change in response to surface contact. A total of 122 genes were determined to have altered transcription between planktonic and surface growth, including several plasmid genes which are involved exopolysaccharide synthesis and the formation of bloom-like aggregates.

中文翻译:

次要菌毛蛋白参与蓝藻集胞藻属的运动和自然能力。PCC 6803

蓝藻合成 IV 型菌毛,已知其对运动、粘附和自然能力至关重要。它们由长而柔韧的纤维组成,主要由集胞藻属中的主要菌毛蛋白 PilA1 组成。PCC 6803。此外,集胞藻编码较少丰富的菌毛蛋白样蛋白,称为次要菌毛蛋白。在这项研究中,我们表明次要菌毛蛋白 PilA5 对于自然转化至关重要,但对于运动和絮凝是可有可无的。相比之下,由pilA9 - slr2019转录单位编码的一组次要菌毛蛋白是运动所必需的,但对于自然转化是可有可无的。pilA5 - pilA6pilA9都不- slr2019对菌毛组装至关重要,因为突变菌株在细胞表面显示 IV 型菌毛。与 PilX 样次要菌毛蛋白相似的另外三种基因产物在集胞藻的絮凝中具有功能。我们的研究结果表明,不同的次要菌毛促进了不同的菌毛功能。此外,我们的微阵列分析表明,次要菌毛蛋白基因的转录水平会随着表面接触而变化。共有 122 个基因被确定为在浮游和表面生长之间发生了改变的转录,其中包括几个涉及胞外多糖合成和绽放样聚集体形成的质粒基因。
更新日期:2021-06-11
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