Control of anthracnose disease caused by Colletotrichum gloeosporioides by use of essential oils encapsulated in nanofibres made of polyvinyl alcohol has been attempted in this study. Essential oils from plants of subtropical origin, thyme oil and betel leaf oil were incorporated in electrospun mats at different levels. The Scanning Electron Microscope analysis revealed size of the nanofibers between 180 and 302 nm. Effective Dose (ED₅₀) values for the pure oils and their blends (1:3; 1:1; 3:1) ranged from 77 to 148 ppm. The blended essential oils demonstrated additive effect for inhibition of the pathogen with Abbott index >0.5. Oil loading capacity, determined using Gas Chromatography Electron Capture Detector ranged from 40.15–75.88% and was found proportional to feed content. No major effect of incorporation of thyme oil and betel oil on polymeric structure was observed in Fourier-transform infrared spectra. A Band near 876cm⁻¹ and 1250 cm⁻¹ signified the presence of both thyme and betel oil respectively. The composite nanofibers with blended oils at three levels showed inhibition zone from 6.6 mm to 11.6 mm on 3rdday of incubation, which increased further upto 24 mm on the 6th day. Sapota fruits inoculated with Colletotrichum gloeosporioidesrecorded disease severity of 83.33 %, 8.39 % and 6.72 % when packed in corrugated fiber board boxes with composite nanofibers as compared to control fruits (100 %) on the 6th day of incubation at 28 °C. Burning symptoms on skin were observed on fruits kept in contact with same level of essential oil infused sterile cotton balls highlighting the importance of encapsulated oils for disease control.

本研究尝试通过使用包裹在由聚乙烯醇制成的纳米纤维中的精油来控制由炭疽病菌引起的炭疽病。来自亚热带植物的精油、百里香油和槟榔叶油以不同的水平掺入电纺垫中。扫描电子显微镜分析显示纳米纤维的尺寸介于 180 和 302 nm 之间。有效剂量（ED ₅₀) 纯油及其混合物 (1:3; 1:1; 3:1) 的值范围为 77 至 148 ppm。混合精油表现出抑制病原体的加成作用，雅培指数 >0.5。使用气相色谱电子捕获检测器测定的油负载能力范围为 40.15-75.88%，发现与进料含量成正比。在傅立叶变换红外光谱中没有观察到百里香油和槟榔油的掺入对聚合物结构的主要影响。876cm ^-1和 1250 cm ^-1附近的波段分别表示百里香和槟榔油的存在。三个水平混合油的复合纳米纤维在孵育第 3 天显示抑制区从 6.6 毫米到 11.6 毫米，并在第 6 天进一步增加到 24 毫米。在 28 °C 下培养的第 6 天，与对照水果 (100%) 相比，接种了胶孢炭疽菌的Sapota 果实在包装在具有复合纳米纤维的瓦楞纸板箱中时记录的疾病严重程度分别为 83.33%、8.39% 和 6.72%。在与注入相同水平精油的无菌棉球接触的水果上观察到皮肤上的燃烧症状，突出了封装油对疾病控制的重要性。