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5-HT neurons of the medullary raphe contribute to respiratory control in toads
Respiratory Physiology & Neurobiology ( IF 2.3 ) Pub Date : 2021-06-10 , DOI: 10.1016/j.resp.2021.103717
Elisa M Fonseca 1 , Carolina R Noronha-de-Souza 2 , Kênia C Bícego 1 , Luiz G S Branco 3 , Luciane H Gargaglioni 1
Affiliation  

Air-breathing vertebrates undergo respiratory adjustments when faced with disturbances in the gas composition of the environment. In mammals, the medullary raphe nuclei are involved in the neuronal pathway that mediates the ventilatory responses to hypoxia and hypercarbia. We investigate whether the serotoninergic neurons of the medullary raphe nuclei of toads (Rhinella diptycha) play a functional role in respiratory control during resting conditions (room air), hypercarbia (5% CO2), and hypoxia (5% O2). The raphe nuclei were located and identified based on the location of the serotoninergic neurons in the brainstem. We then lesioned the medullary raphe (raphe pallidus, obscurus and magnus) with anti-SERT-SAP and measured ventilation in both control and lesioned groups and we observed that serotonin (5-HT) specific chemical lesions of the medullary raphe caused reduced respiratory responses to both hypercarbia and hypoxia. In summary, we report that the serotoninergic neurons of the medullary raphe of the cururu toad Rhinella diptycha participate in the chemoreflex responses during hypercarbia and hypoxia, but not during resting conditions. This current evidence in anurans, together with the available data in mammals, brings insights to the evolution of brain sites, such as the medullary raphe, involved in the ventilatory chemoreflex in vertebrates.



中文翻译:

中缝的 5-HT 神经元有助于蟾蜍的呼吸控制

呼吸空气的脊椎动物在面临环境气体成分的干扰时会进行呼吸调整。在哺乳动物中,中缝髓核参与介导对缺氧和高碳酸血症的通气反应的神经元通路。我们研究了蟾蜍(Rhinella diptycha)中缝髓核的血清素能神经元是否在静息条件(室内空气)、高碳酸血症(5% CO 2)和缺氧(5% O 2)。根据脑干中血清素能神经元的位置定位和识别中缝核。然后,我们用抗 SERT-SAP 损伤中缝(苍白中缝、暗缝和大缝)并测量对照组和损伤组的通气量,我们观察到中缝的血清素 (5-HT) 特异性化学损伤导致呼吸反应减少高碳酸血症和缺氧。总之,我们报告了 cururu toad Rhinella diptycha髓中缝的 5-羟色胺能神经元在高碳酸血症和缺氧期间参与化学反射反应,但在静息条件下不参与。目前在无尾类动物中的证据以及哺乳动物中的可用数据为大脑部位的进化带来了见解,例如参与脊椎动物通气化学反射的髓中缝。

更新日期:2021-06-16
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