The study aimed to investigate the effects of vitamin E injection for the prevention of transport stress on ewes. Kivircik ewes (2–3 years old, n = 24) were randomly separated into three groups; G1 (Control) and G2 treated with 14 ml. saline as the placebo, G3 treated with 2100 IU/ind. DL-alpha-tocopherol acetate prior to transport. G2 and G3 were transported at 80 km/h for 4 h on a truck. Serum samples were obtained before (T0) and after (T1) transport. Serum cortisol, catalase, IgG, ceruloplasmin, C-reactive protein, complement component 4, interleukin-1 beta, tumour necrosis factor-alpha, glutathione peroxidase (GPx), superoxide dismutase, malondialdehyde analyses performed by ELISA, and serum alpha-tocopherol concentrations were evaluated by HPLC-UV. Wilcoxon and Kruskal–Wallis tests were used for statistical assessments (p < 0.05). Alpha-tocopherol concentrations were founded 1.22 ± 0.82, 0.27 ± 0.14 and 0.14 ± 0.07 µmol/L, respectively, in G1, G2 and G3 at T1. Alpha-tocopherol concentration decreased significantly in G2 between T0 and T1. GPx concentrations were increased twofold in G2 and G3 between T0 and T1 (p < 0.01). As a result, G2 alpha-tocopherol concentrations decreased but, the stress and oxidative parameters tested in this study were not affected by treating 2100 IU/ind. DL-alpha-tocopherol acetate before transport.

中文翻译：

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维生素 E 处理对遭受运输应激的 Kivircik 母羊选定的免疫和氧化参数的影响

该研究旨在调查维生素 E 注射对预防母羊运输应激的影响。Kivircik 母羊（2-3 岁，n = 24) 随机分为三组；G1（对照）和 G2 用 14 ml 处理。生理盐水作为安慰剂，G3 用 2100 IU/ind 治疗。运输前的 DL-α-生育酚乙酸酯。G2 和 G3 用卡车以 80 公里/小时的速度运输 4 小时。在 (T0) 运输之前和 (T1) 运输之后获得血清样品。血清皮质醇、过氧化氢酶、IgG、血浆铜蓝蛋白、C 反应蛋白、补体成分 4、白细胞介素 1 β、肿瘤坏死因子 α、谷胱甘肽过氧化物酶 (GPx)、超氧化物歧化酶、ELISA 进行的丙二醛分析和血清 α-生育酚浓度通过 HPLC-UV 进行评估。Wilcoxon 和 Kruskal-Wallis 检验用于统计评估（p < 0.05)。在 T1 的 G1、G2 和 G3 中，α-生育酚浓度分别为 1.22 ± 0.82、0.27 ± 0.14 和 0.14 ± 0.07 µmol/L。α-生育酚浓度在 G2 中在 T0 和 T1 之间显着降低。GPx 浓度在 G2 和 G3 在 T0 和 T1 之间增加了两倍（p  < 0.01）。因此，G2 α-生育酚浓度降低，但本研究中测试的压力和氧化参数不受治疗 2100 IU/ind 的影响。运输前的 DL-α-生育酚醋酸酯。