The global fight against mosquito-borne viral diseases has in recent years been bolstered by the introduction of the endosymbiotic bacteria Wolbachia to vector populations, which in host mosquitoes supresses the transmissibility of several viruses. Researchers engaged on this front of the battle often need to know the Wolbachia infection status of individual mosquitoes as the intervention progresses and the mosquitoes become established in the target population. Previously we have successfully applied ATR-FTIR spectroscopy to the detection of Wolbachia in adult <i>Aedes aegypti</i> mosquitoes; here we apply the same principles to Aedes eggs, with sensitivity and selectivity > 0.95. Further, we successfully distinguish between infections in eggs of the wMel and wMelPop strains of <i>Wolbachia pipientis</i>, with a classification error of 3%. The disruption of host lipid profile by Wolbachia is found to be a key driver in spectral differences between these sample classes.

近年来，通过将内共生细菌沃尔巴克氏体引入载体种群，全球对抗蚊媒病毒疾病的斗争得到了加强，这在宿主蚊子中抑制了几种病毒的传播。随着干预的进行和蚊子在目标人群中的建立，从事这一战的研究人员通常需要了解单个蚊子的沃尔巴克氏菌感染状态。此前，我们已成功地将 ATR-FTIR 光谱应用于埃及伊蚊成年蚊子的沃尔巴克氏体检测；在这里，我们将相同的原理应用于伊蚊卵，灵敏度和选择性 > 0.95。此外，我们成功区分了 <i>Wolbachia pipientis</i> 的 wMel 和 wMelPop 菌株的鸡蛋感染，分类误差为 3%。发现 Wolbachia 对宿主脂质谱的破坏是这些样本类别之间光谱差异的关键驱动因素。