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Difenoconazole induces oxidative DNA damage and mitochondria mediated apoptosis in SH-SY5Y cells
Chemosphere ( IF 8.8 ) Pub Date : 2021-06-10 , DOI: 10.1016/j.chemosphere.2021.131160
Xin Wang 1 , Hongfei Ni 1 , Wenping Xu 1 , Bing Wu 1 , Te Xie 1 , Cheng Zhang 2 , Jiagao Cheng 1 , Zhong Li 1 , Liming Tao 1 , Yang Zhang 1
Affiliation  

Difenoconazole is one of the most typical triazole fungicides. Difenoconazole is widely used in the field of agricultural production, and its health and safety problems need to be further studied. The main purpose of this paper is to verify the neurotoxicity of Difenoconazole at the cellular level. In this study, SH-SY5Y cell line of human neuroblastoma was used to evaluate its potentially toxic effects and molecular mechanism in vitro. The research indicated that Difenoconazole could reduce cell viability and inhibit cell proliferation, induce DNA damage and accelerate programmed cell death. Further studies showed that Difenoconazole induced DNA double-strand breaks, intracellular generation of ROS, cleaved PARP, mitochondrial membrane potential collapse, induced Cyt c release, and Bax/Bcl-2 ratio increase in SH-SY5Y cells. In conclusion, the cytotoxicity of Difenoconazole revealed its toxic effect on SH-SY5Y cells, and the IC50 value was 55.41 μM after 24 h exposure. Meanwhile, the genetic toxicity of Difenoconazole has revealed that it can induce DNA damage and apoptosis of SH-SY5Y cells. Through this study, the toxic effects of Difenoconazole on SH-SY5Y cells are further understood, which provides a more scientific basis for its safe use and risk control.



中文翻译:

苯醚甲环唑在 SH-SY5Y 细胞中诱导氧化性 DNA 损伤和线粒体介导的细胞凋亡

苯醚甲环唑是最典型的三唑类杀菌剂之一。苯醚甲环唑广泛应用于农业生产领域,其健康安全问题有待进一步研究。本文的主要目的是在细胞水平上验证苯醚甲环唑的神经毒性。本研究采用人神经母细胞瘤SH-SY5Y细胞系体外评价其潜在毒性作用和分子机制。研究表明苯醚甲环唑可降低细胞活力,抑制细胞增殖,诱导DNA损伤,加速细胞程序性死亡。进一步研究表明苯醚甲环唑诱导DNA双链断裂、细胞内ROS产生、PARP裂解、线粒体膜电位崩溃、诱导Cyt cSH-SY5Y 细胞中的释放和 Bax/Bcl-2 比率增加。总之,苯醚甲环唑的细胞毒性揭示了其对SH-SY5Y细胞的毒性作用,暴露24小时后IC 50值为55.41 μM。同时,苯醚甲环唑的遗传毒性表明它可以诱导SH-SY5Y细胞的DNA损伤和凋亡。通过本研究,进一步了解苯醚甲环唑对SH-SY5Y细胞的毒性作用,为其安全使用和风险控制提供更科学的依据。

更新日期:2021-06-15
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