Fresnel incoherent correlation holography technology can not only combine other techniques for three-dimensional imaging of objects but also has the inherent ability to create a super-resolution reconstruction. It generally realizes reconstructions of objects through phase-shifting algorithm, so it is not appropriate for the real-time imaging of living cells. We propose a single-shot microscopic imaging method, which adjusts the pixel distribution of the SLM based on a two-step phase-shifted mask to make sure beams can travel along their respective optical axis, then the multiplexed phase-shifted hologram can be obtained simultaneously. Experimental reconstructions of some objects are used to verify the effectiveness of our proposal. It is hopeful to realize the real-time imaging of living cells by using this method.

菲涅尔非相干相关全息技术不仅可以结合其他技术对物体进行三维成像，而且具有创造超分辨率重建的内在能力。它一般通过相移算法实现物体的重建，因此不适用于活细胞的实时成像。我们提出了一种单次显微成像方法，该方法基于两步相移掩模调整SLM的像素分布，以确保光束可以沿着各自的光轴传播，然后可以获得复用相移全息图同时地。一些对象的实验重建用于验证我们建议的有效性。该方法有望实现活细胞的实时成像。