Purpose

Based on DNase I and reduced graphene oxide (rGO)-magnetic silicon microspheres (MNPS), a highly sensitive and selective fluorescent probe for the detection of PD-L1 was developed.

Design/methodology/approach

Here °C we present a feasibility of biosensor to detection of PD-L1 in lung tumors plasma. In the absence of PD-L1°C the PD-L1 aptamer is absorbed on the surface of graphene oxide modified magnetic nanoparticles °8rGO-MNPS°9 and leading to effective fluorescence quenching. Upon adding PD-L1°C the aptamer sequences could be specifically recognized by PD-L1 and the aptamer/PD-L1 complex is formed°C resulting in the recovery of quenched fluorescence.

Findings

This sensor can detect PD-L1 with a linear range from 100 pg mL⁻¹ to 100 ng mL⁻¹, and a detection limit of 10 pg•m⁻¹ was achieved.

Originality/value

This method provides an easy and sensitive method for the detection of PD-L1 and will be beneficial to the early diagnosis and prognosis of tumors.

中文翻译：

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利用 DNase I 和氧化石墨烯修饰的磁性纳米粒子来检测人血浆中的 PD-L1

目的

基于 DNase I 和还原氧化石墨烯 (rGO)-磁性硅微球 (MNPS)，开发了一种用于检测 PD-L1 的高灵敏度和选择性荧光探针。

设计/方法/方法

在这里°C 我们提出了生物传感器检测肺肿瘤血浆中 PD-L1 的可行性。在不存在 PD-L1°C 的情况下，PD-L1 适配体被吸附在氧化石墨烯修饰的磁性纳米粒子°8rGO-MNPS°9 的表面上，并导致有效的荧光猝灭。在加入 PD-L1°C 后，适配体序列可以被 PD-L1 特异性识别，适配体/PD-L1 复合物形成°C，导致淬灭荧光的恢复。

发现

该传感器可以在100 pg mL ^-1至100 ng mL ^-1的线性范围内检测PD-L1 ，并达到10 pg•m ^-1的检测限。

原创性/价值

该方法为PD-L1的检测提供了一种简便、灵敏的方法，将有利于肿瘤的早期诊断和预后判断。