ABSTRACT

MYB106 and MYB16 are MIXTA-like transcription factors that control trichome maturation and cuticle formation in Arabidopsis. In a recent study, we found that the TEOSINTE BRANCHED 1, CYCLOIDEA and PROLIFERATING CELL FACTORS (TCP) transcription factor TCP15 also acts as an important regulator of aerial epidermis specialization in Arabidopsis through the control of trichome development and cuticle formation. TCP15 and MYB106 regulate the expression of common groups of genes, including genes coding for transcription factors and enzymes of the cuticle biosynthesis pathway. In this study, we report that TCP15 physically interacts with MYB106 when both proteins are expressed in yeast cells or Nicotiana bentamiana leaves. Furthermore, we also observed interaction in leaves of Arabidopsis thaliana. Altogether, our findings raise the possibility that TCP15 and MYB106 bind together to the promoters of target genes to exert their action. Our data provide a base to investigate the role of TCP-MIXTA complexes in the context of cuticle development in Arabidopsis thaliana.

摘要

MYB106 和 MYB16 是控制拟南芥毛状体成熟和角质层形成的 MIXTA 样转录因子。在最近的一项研究中，我们发现 TEOSINTE BRANCHED 1、环类和增殖细胞因子 (TCP) 转录因子 TCP15 还通过控制毛状体发育和角质层形成作为拟南芥气生表皮特化的重要调节因子。TCP15 和 MYB106 调节常见基因组的表达，包括编码转录因子和角质层生物合成途径酶的基因。在这项研究中，我们报告说，当 TCP15 和 MYB106 都在酵母细胞或烟草中表达时，TCP15 与 MYB106 发生物理相互作用。树叶。此外，我们还观察到拟南芥叶片中的相互作用。总之，我们的研究结果提出了 TCP15 和 MYB106 与靶基因的启动子结合以发挥其作用的可能性。我们的数据为研究 TCP-MIXTA 复合物在拟南芥角质层发育中的作用提供了基础。