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Core-shell PLA/Kef hybrid scaffolds for skin tissue engineering applications prepared by direct kefiran coating on PLA electrospun fibers optimized via air-plasma treatment
Biomaterials Advances ( IF 7.9 ) Pub Date : 2021-06-10 , DOI: 10.1016/j.msec.2021.112248
Francesco Lopresti 1 , Simona Campora 2 , Giorgia Tirri 1 , Elisa Capuana 1 , Francesco Carfì Pavia 1 , Valerio Brucato 1 , Giulio Ghersi 3 , Vincenzo La Carrubba 4
Affiliation  

Over the recent years, there is a growing interest in electrospun hybrid scaffolds composed of synthetic and natural polymers that can support cell attachment and proliferation. In this work, the physical and biological properties of polylactic acid (PLA) electrospun mats coated with kefiran (Kef) were evaluated. Gravimetric, spectroscopic (FTIR-ATR) and morphological investigations via scanning electron microscopy confirmed the effective formation of a thin kefiran layer wrapped on the PLA fibers with an easy-tunable thickness. Air plasma pre-treatment carried out on PLA (P-PLA) affected both the morphology and the crystallinity of Kef coating as confirmed by differential scanning calorimetry and X-ray diffraction analyses. Scaffolds were mechanically characterized with tensile tests to evaluate the reinforcing action of the Kef coating. The water resistance of Kefiran coating in distilled water at 37 °C evaluated on both PLA/Kef and P-PLA/Kef was carried out by gravimetric and morphological analyses. Finally, cell culture assays with embryonic fibroblast cells were conducted on selected hybrid scaffolds to compare the cell proliferation, morphology, and collagen production with PLA and P-PLA electrospun scaffolds. Based on the results, we can demonstrate that direct coating of PLA from Kef/water solutions is an effective approach to prepare hybrid scaffolds with tunable properties and that the plasma pre-treatment enhances the affinity between PLA and Kefiran. In vitro tests demonstrated the great potential of PLA/Kef hybrid scaffolds for skin tissue engineering.



中文翻译:

用于皮肤组织工程应用的核壳 PLA/Kef 混合支架通过空气等离子体处理优化的 PLA 电纺纤维上的直接开菲兰涂层制备

近年来,人们对由合成和天然聚合物组成的电纺混合支架越来越感兴趣,这些支架可以支持细胞附着和增殖。在这项工作中,评估了涂有开菲兰 (Kef) 的聚乳酸 (PLA) 电纺垫的物理和生物学特性。通过扫描电子显微镜进行的重量、光谱 (FTIR-ATR) 和形态研究证实了包裹在 PLA 纤维上的薄凯菲兰层的有效形成,其厚度易于调节。差示扫描量热法和 X 射线衍射分析证实,对 PLA (P-PLA) 进行的空气等离子体预处理会影响 Kef 涂层的形态和结晶度。通过拉伸试验对支架进行机械表征,以评估 Kef 涂层的增强作用。通过重量分析和形态分析,对 PLA/Kef 和 P-PLA/Kef 上的 Kefiran 涂层在 37°C 蒸馏水中的耐水性进行了评估。最后,在选定的混合支架上进行胚胎成纤维细胞的细胞培养测定,以比较 PLA 和 P-PLA 电纺支架的细胞增殖、形态和胶原蛋白生成。基于这些结果,我们可以证明从 Kef/水溶液中直接涂覆 PLA 是制备具有可调特性的混合支架的有效方法,并且等离子体预处理增强了 PLA 和 Kefiran 之间的亲和力。体外测试证明了 PLA/Kef 混合支架在皮肤组织工程中的巨大潜力。

更新日期:2021-06-16
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