Abstract

In addition to the transmission of paternal genome, spermatozoa also carry coding as well as noncoding microRNAs (miRNAs) into the female oocyte during the process of biological fertilization. Based on RNA deep sequencing, a total 28 number of differentially expressed miRNAs were cataloged in categorized Frieswal^TM crossbred (Holstein Friesian X Sahiwal) bull semen on the basis of conception rate (CR) in field progeny testing program. Validation of selected miRNAs viz. bta-mir-182, bta-let-7b, bta-mir-34c and bta-mir-20a revealed that, superior bull semen having comparatively (p < .05) lower level of all the miRNAs in contrast to inferior bull semen. Additionally, it was illustrated that, bta-mir-20a and bta-mir-34c miRNAs are negatively (p < .01) correlated with seminal plasma catalase (CAT) activity and glutathione peroxidase (GPx) level. Interactome studies identified that bta-mir-140, bta-mir-342, bta-mir-1306 and bta-mir-217 can target few of the important solute carrier (SLC) proteins viz. SLC30A3, SLC39A9, SLC31A1 and SLC38A2, respectively. Interestingly, it was noticed that all the SLCs were significantly (p < .05) expressed at higher level in superior quality bull semen and they are negatively correlated (p < .01) with their corresponding miRNAs as mentioned. This study may reflect the role of miRNAs in regulating few of the candidate genes and thus may influence the bull semen quality traits.

摘要

除了父系基因组的传递外，精子还在生物受精过程中携带编码和非编码 microRNA (miRNA) 进入雌性卵母细胞。基于 RNA 深度测序，在野外后代测试程序中，根据受孕率 (CR)，在分类的 Frieswal ^{TM杂交 (Holstein Friesian X Sahiwal) 公牛精液中对总共 28 个差异表达的 miRNA 进行了分类。}验证选定的 miRNA 即。bta-mir-182、bta-let-7b、bta-mir-34c 和 bta-mir-20a 显示，与劣质公牛精液相比，优质公牛精液具有相对 ( p  < .05) 较低水平的所有 miRNA。此外，还表明，bta-mir-20a 和 bta-mir-34c miRNA 呈负性 ( p < .01) 与精浆过氧化氢酶 (CAT) 活性和谷胱甘肽过氧化物酶 (GPx) 水平相关。Interactome 研究发现，bta-mir-140、bta-mir-342、bta-mir-1306 和 bta-mir-217 可以靶向少数重要的溶质载体 (SLC) 蛋白，即。分别为 SLC30A3、SLC39A9、SLC31A1 和 SLC38A2。有趣的是，注意到所有 SLC 在优质公牛精液中均以较高水平显着 ( p < .05) 表达，并且它们与它们相应的 miRNA 呈负相关 ( p  < .01)，如前所述。这项研究可能反映了 miRNAs 在调节少数候选基因中的作用，从而可能影响公牛精液质量性状。