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Dynamic Changes of Metabolite Profiles in Maternal Biofluids during Gestation Period in Huanjiang Mini-pigs
Frontiers in Veterinary Science ( IF 3.2 ) Pub Date : 2021-06-10 , DOI: 10.3389/fvets.2021.636943
Qian Zhu 1, 2 , Peifeng Xie 1 , Huawei Li 1 , Francois Blachier 3 , Yulong Yin 1 , Xiangfeng Kong 1, 4
Affiliation  

The biochemical parameters related to nitrogenous metabolism in maternal biofluids may be linked and even reflect the fetal metabolism and growth. The present study have measured the concentrations of various parameters related to amino acid (AA) and lipid metabolism, as well as different metabolites including the free AAs in maternal plasma and amniotic and allantoic fluid corresponding to fetuses with different body weight (BW) during different gestation period, in order to identify the possible relationships between biochemical parameters and fetal growth. A total of 24 primiparous Huanjiang mini-pigs were fed with a standard diet. Data showed that, from day 45 to 110 of gestation, the maternal plasma levels of alanine aminotransferase (ALT), albumin (ALB), Ile, Orn, Car, α-ABA, and β-AiBA increased (P < 0.05); while the levels of ammonia (AMM), choline esterase (CHE), high density lipoprotein-cholesterol (HDL-C), Leu, Glu, Cys, Asp, and Hypro decreased (P < 0.05). From day 45 to 110 of gestation, the amniotic fluid levels of aspartate transaminase (AST), CHE, total protein (TP), and urea nitrogen (UN) increased (P < 0.05), as well as the level of CHE and TP and concentration of Pro in allantoic fluid; while the amniotic fluid concentrations of Arg, Glu, Orn, Pro, and Tau decreased (P < 0.05), as well as allantoic fluid concentrations of Arg and Glu. At day 45 of gestation, the amniotic fluid concentrations of Arg, Orn, and Tau corresponding to the highest BW (HBW) fetuses were higher (P < 0.05), whereas the allantoic fluid concentrations of His and Pro were lower (P < 0.05) when compared with the lowest BW (LBW) fetuses. At day 110 of gestation, the amniotic fluid concentration of Tau corresponding to the HBW fetuses was higher (P < 0.05) than the LBW fetuses. These findings show that the sows display increased protein utilization and decreased lipid metabolism and deposition from day 75 to 110 of gestation. In addition, our data are indicative of a likely stronger ability of HBW fetuses to metabolize protein; and finally of a possible key role of Arg, Gln, Glu, Pro, Tau, and His for the fetal growth and development.

中文翻译:

环江小型猪妊娠期母体体液代谢物谱的动态变化

母体体液中与氮代谢相关的生化参数可能与胎儿的代谢和生长有关,甚至可以反映胎儿的代谢和生长。本研究测量了与氨基酸 (AA) 和脂质代谢相关的各种参数的浓度,以及不同代谢物的浓度,包括不同体重 (BW) 胎儿在母体血浆、羊水和尿囊液中的游离氨基酸。妊娠期,以确定生化参数与胎儿生长之间可能的关系。24头初产环江小型猪饲喂标准日粮。数据显示,妊娠第45~110天,母体血浆丙氨酸转氨酶(ALT)、白蛋白(ALB)、Ile、Orn、Car、α-ABA和β-AiBA水平升高(P < 0.05);而氨(AMM)、胆碱酯酶(CHE)、高密度脂蛋白胆固醇(HDL-C)、Leu、Glu、Cys、Asp和Hypro的水平降低(P < 0.05)。妊娠第45~110天,羊水中天冬氨酸转氨酶(AST)、CHE、总蛋白(TP)、尿素氮(UN)升高(P < 0.05),CHE、TP和尿囊液中 Pro 的浓度;而羊水中 Arg、Glu、Orn、Pro 和 Tau 的浓度降低(P < 0.05),以及尿囊液中 Arg 和 Glu 的浓度。在妊娠第 45 天,与最高 BW (HBW) 胎儿对应的 Arg、Orn 和 Tau 的羊水浓度较高(P < 0.05),而 His 和 Pro 的尿囊液浓度较低(P < 0.05)与最低体重 (LBW) 的胎儿相比。在妊娠第 110 天,HBW 胎儿对应的羊水中 Tau 浓度高于 LBW 胎儿(P < 0.05)。这些发现表明,母猪在妊娠 75 至 110 天期间蛋白质利用增加,脂质代谢和沉积减少。此外,我们的数据表明 HBW 胎儿可能具有更强的蛋白质代谢能力;最后是 Arg、Gln、Glu、Pro、Tau 和 His 对胎儿生长发育的可能关键作用。我们的数据表明 HBW 胎儿可能具有更强的蛋白质代谢能力;最后是 Arg、Gln、Glu、Pro、Tau 和 His 对胎儿生长发育的可能关键作用。我们的数据表明 HBW 胎儿可能具有更强的蛋白质代谢能力;最后是 Arg、Gln、Glu、Pro、Tau 和 His 对胎儿生长发育的可能关键作用。
更新日期:2021-06-10
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