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The complete mitochondrial genome of Glycyphagus domesticus (Acari: Glycyphagidae) using next-generation sequencing: Insight into phylogeny of Acariformes
Journal of Stored Products Research ( IF 2.7 ) Pub Date : 2021-06-09 , DOI: 10.1016/j.jspr.2021.101818
Yangming Lan , Shiqian Feng , Vaclav Stejskal , Yi Wu , Radek Aulicky , Yang Cao , Zhihong Li

Glycyphagus domesticus (Acari: Glycyphagidae) is a pest mite with worldwide distribution. It damages stored products and produces allergens. Molecular diagnosis is important for the effective monitoring and control of stored-products and dust pest mites. The complete mitochondrial genome (mt genome) has become one of the most useful markers for molecular systematics, phylogenetics and diagnostics of stored-pest mites. In this study, next-generation sequencing was used to sequence the complete mt genome of G. domesticus. The complete mt genome was found to be 14,333 bp in size, containing 13 protein coding genes, 2 ribosomal RNA (rRNA) genes and 22 transfer RNA (tRNA) genes. We constructed phylogenetic trees based on concatenated thirteen protein-coding genes and two rRNAs genes. Our overall results showed that G. domesticus was found in its own separate clade from the 29 other mite species, which is likely due to the fact that this represents the first assembled mt genome of a member of the family Glycyphagidae. Overall, our study enriches the growing mt genome database for the Acariformes and will promote further studies.



中文翻译:

的线粒体基因组全ģ lycyphagus domesticus(蜱螨亚纲:食甜螨科),使用下一代测序:洞察真螨目的系统发育

家蝇(Acari:Glycyphagidae)是一种分布于世界各地的害虫。它会损坏储存的产品并产生过敏原。分子诊断对于有效监测和控制储存产品和尘螨具有重要意义。完整的线粒体基因组(mt 基因组)已成为对储藏害虫的分子系统学、系统发育学和诊断学最有用的标记之一。在这项研究中,使用下一代测序测序的完整基因组的MT G. domesticus. 发现完整的 mt 基因组大小为 14,333 bp,包含 13 个蛋白质编码基因、2 个核糖体 RNA (rRNA) 基因和 22 个转移 RNA (tRNA) 基因。我们构建了基于串联的 13 个蛋白质编码基因和两个 rRNAs 基因的系统发育树。我们的总体结果表明,G.domesticus是在与其他 29 种螨类不同的单独进化枝中发现的,这可能是因为这代表了 Glycyphagidae 家族成员的第一个组装的 mt 基因组。总的来说,我们的研究丰富了不断增长的螨类基因组数据库,并将促进进一步的研究。

更新日期:2021-06-09
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