This research was aimed to understand the effects of extraction temperature, liquid-to-solid ratio, and ethanol concentration of an ethanol–water solvent system on crude antioxidants obtained from edible macroalgae Ascophyllum nodosum. The total phenol content (TPC), total carbohydrate content (TCC), and DPPH radical scavenging assays were measured for both the fresh extract (FE) and dried extract (DE). The highest TPC in FE was observed at 30 °C, 90 mL/g, and 100% ethanol concentration. The TCC of FE tended to increase with increasing temperature and decreasing liquid-to-solid ratio, while moderate ethanol concentration (40%) resulted in the highest TCC. The strongest antioxidant activity of the FE was observed at low temperatures (30 or 40 °C), relatively low liquid-to-solid ratio (30 mL/g), and high ethanol concentration (80 or 100%). All DE, except for those obtained at 60 or 70 °C, showed lower TPC, TCC, and DPPH radical scavenging activity than their FE counterparts.

中文翻译：

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使用二元溶剂萃取系统从褐藻泡叶藻中提取抗氧化剂

本研究旨在了解提取温度、液固比和乙醇-水溶剂体系的乙醇浓度对从食用大型藻类泡叶藻中提取的粗抗氧化剂的影响。. 测量新鲜提取物 (FE) 和干提取物 (DE) 的总酚含量 (TPC)、总碳水化合物含量 (TCC) 和 DPPH 自由基清除测定。在 30 °C、90 mL/g 和 100% 乙醇浓度下观察到 FE 中的最高 TPC。FE 的 TCC 倾向于随着温度的升高和液固比的降低而增加，而中等浓度的乙醇（40%）导致最高的 TCC。在低温（30 或 40 °C）、相对较低的液固比（30 mL/g）和高乙醇浓度（80 或 100%）下观察到 FE 的最强抗氧化活性。除了在 60 或 70 °C 获得的那些外，所有 DE 都显示出比它们的 FE 对应物更低的 TPC、TCC 和 DPPH 自由基清除活性。