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Association of KCTD15 gene with fat deposition in pigs
Journal of Animal Physiology and Animal Nutrition ( IF 2.7 ) Pub Date : 2021-06-09 , DOI: 10.1111/jpn.13587
Pan Zhang 1 , Yu Fu 1 , Ran Zhang 1 , Peng Shang 2 , Hao Zhang 1 , Bo Zhang 1
Affiliation  

KCTD15 is associated with body mass index and fat deposition in humans, mice and chickens. However, the function of KCTD15 in pig fat deposition remains unclear. In this study, we cloned and analysed the cDNA sequence of porcine KCTD15. The full length of the mRNA sequence of KCTD15 is 4,091 bp, encoding 283 amino acids. The protein is hydrophilic, it has a relative molecular mass of about 31.9 kDa and an isoelectric point of 7.09 with no signal peptide sequence or transmembrane structure. Expression analysis showed that KCTD15 expression level was significantly higher in the tissues of Large White pigs (LW) than in those of Tibetan pigs (TP) and Diannan Small-ear pigs (DN) at 6 months of age, whereas its expression level in embryonic tissues of LW at 60 days was lower than that in tissues of TP and Wujin pigs (WJ). In pig primary adipocytes, the expression level of KCTD15 is high in the early stage of differentiation and gradually decreases in later stages. Additionally, the single-nucleotide polymorphism (SNP) site T-2030C (T/C mutation, located 2,030 bp upstream of the start codon) showed a dominant allele T with high promoter activity in the LW population and a dominant allele C in the TP and WJ populations. Our results indicate that KCTD15 is involved in pig fat deposition and that T-2030C is an important regulatory site for transcriptional activity, affecting fat deposition.

中文翻译:

KCTD15基因与猪脂肪沉积的关系

KCTD15与人类、小鼠和鸡的体重指数和脂肪沉积有关。然而,KCTD15在猪脂肪沉积中的作用仍不清楚。在本研究中,我们克隆并分析了猪KCTD15的cDNA序列。KCTD15的mRNA序列全长4091 bp,编码283个氨基酸。该蛋白具有亲水性,相对分子质量约为31.9 kDa,等电点为7.09,无信号肽序列或跨膜结构。表达分析表明,KCTD156月龄大白猪(LW)组织中的表达水平显着高于藏猪(TP)和滇南小耳猪(DN),而在60岁时LW胚胎组织中的表达水平显着升高。天数低于 TP 和武进猪 (WJ) 的组织。在猪原代脂肪细胞中, KCTD15的表达水平在分化早期高,后期逐渐降低。此外,单核苷酸多态性 (SNP) 位点 T-2030C(T/C 突变,位于起始密码子上游 2,030 bp)在 LW 群体中显示出具有高启动子活性的显性等位基因 T,在 TP 中显示出显性等位基因 C和 WJ 人口。我们的结果表明,K CTD15参与猪脂肪沉积,并且 T-2030C 是转录活性的重要调节位点,影响脂肪沉积。
更新日期:2021-06-09
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