Two-phase partitioning bioreactors (TPPBs) have been extensively used for volatile organic compounds (VOCs) removal. To date, most studies have focused on improving the mass transfer of gas phases/non-aqueous phases (NAPs)/aqueous phases, whereas the NAP/biological phases and gas/biological phases transfer has been neglected. Herein, chitosan was introduced into a TPPB to increase cell surface hydrophobicity (CSH) and improve the n-hexane mass transfer. The performance and stability of the TPPB with chitosan for n-hexane biodegradation were investigated, and it was found out that the TPPB with chitosan achieved maximum removal efficiency and elimination capacity of 80.6% and 26.5 g m⁻³ h⁻¹, thereby reaching much higher values than those obtained without chitosan (61.3% and 15.2 g m⁻³ h⁻¹). Chitosan not only obvio usly increased cell surface hydrophobicity and cell dry biomass on the surface of silicone oil, but might also allow hydrophobic cells in aqueous phases to directly capture and biodegrade n-hexane, resulting in an obvious improvement of mass transfer from the gas phase to biomass. Stability enhancement was another attractive advantage from chitosan addition. This study might provide a new strategy for the development of TPPB in the hydrophobic VOCs treatment.

两相分配生物反应器 (TPPB) 已广泛用于去除挥发性有机化合物 (VOC)。迄今为止，大多数研究都集中在改善气相/非水相 (NAP)/水相的传质上，而忽略了 NAP/生物相和气相/生物相转移。在本文中，脱乙酰壳多糖引入到TPPB以增加表面疏水性（CSH），提高了Ñ正己烷质量传递。的性能和与脱乙酰壳多糖为TPPB的稳定性Ñ己烷生物降解进行了研究，并且发现，随着脱乙酰壳多糖的TPPB能够最大限度地去除效率和80.6％的净化能力和26.5克间^-3 ħ ^-1，从而达到比没有壳聚糖时获得的值高得多的值（61.3% 和 15.2 g m ^-3 h ^-1）。脱乙酰壳多糖不仅obvio usly增加细胞表面的疏水性和细胞干生物量硅油的表面上，但也可能允许水相的疏水性细胞直接捕获和生物降解Ñ己烷，导致从气相传质的明显改善到生物质。稳定性增强是壳聚糖添加的另一个吸引人的优势。该研究可能为TPPB在疏水性VOCs处理中的发展提供新的策略。