Numerous studies have found that microRNAs (miRNAs) are involved in regulating various tumor-related biological functions. The downregulation of miR185-3p have been identified in various types of cancer but the effect and its underlying molecular mechanism in cervical cancer have not been elucidated. Therefore, it is important to investigate the role of miRNAs associated with cervical cancer and its corresponding molecular mechanism to develop new therapeutic targets. The cell counting kit (CCK-8) assay was performed to measure the cell viability. The quantitative real-time PCR (qRT-PCR) and western blot analyses were carried out to identify mRNA and protein expression levels, respectively. Besides, a luciferase activity assay was conducted to confirm the target miRNA gene predictions. In this study, it is found that miR185-3p expression was potentially downregulated in cervical cancer tissues when compared with normal tissues. The CCK-8 results indicated that miR185-3p overexpression suppressed the cancer cell proliferation and the downregulation of miR185-3p enhanced the cancer cell growth. Further, enhanced miR185-3p expression led to a reduction in Annexin-A8 (Anx-A8) expression but miR185-3p inhibition promoted ANX-A8 levels in cervical cancer cells. The luciferase reporter assay indicated that ANX-A8 was a direct target of miR185-3p in cervical cancer cells.

大量研究发现，microRNAs (miRNAs) 参与调节各种与肿瘤相关的生物学功能。miR185-3p 的下调已在各种类型的癌症中被发现，但其在宫颈癌中的作用及其潜在的分子机制尚未阐明。因此，研究与宫颈癌相关的miRNA的作用及其相应的分子机制对于开发新的治疗靶点具有重要意义。进行细胞计数试剂盒（CCK-8）测定以测量细胞活力。进行定量实时 PCR (qRT-PCR) 和蛋白质印迹分析以分别鉴定 mRNA 和蛋白质表达水平。此外，进行荧光素酶活性测定以确认靶miRNA基因预测。在这项研究中，发现与正常组织相比，宫颈癌组织中miR185-3p的表达可能下调。CCK-8结果表明miR185-3p过表达抑制癌细胞增殖，miR185-3p下调促进癌细胞生长。此外，增强的 miR185-3p 表达导致 Annexin-A8 (Anx-A8) 表达降低，但 miR185-3p 抑制促进了宫颈癌细胞中的 ANX-A8 水平。荧光素酶报告基因检测表明 ANX-A8 是宫颈癌细胞中 miR185-3p 的直接靶标。增强的 miR185-3p 表达导致 Annexin-A8 (Anx-A8) 表达降低，但 miR185-3p 抑制促进了宫颈癌细胞中的 ANX-A8 水平。荧光素酶报告基因检测表明 ANX-A8 是宫颈癌细胞中 miR185-3p 的直接靶标。增强的 miR185-3p 表达导致 Annexin-A8 (Anx-A8) 表达降低，但 miR185-3p 抑制促进了宫颈癌细胞中的 ANX-A8 水平。荧光素酶报告基因检测表明 ANX-A8 是宫颈癌细胞中 miR185-3p 的直接靶标。