Industrial lignin such as kraft lignin is an abundant feedstock for renewable chemicals and materials. In this study, a process was developed for depolymerization of kraft lignin followed by an upgrading separation step and further bioconversion of the obtained monoaromatic compounds to muconic acid. First, industrial kraft lignin, Indulin AT, was processed into a guaiacol-rich stream using base-catalyzed depolymerization. This stream was subsequently upgraded using liquid–liquid extraction and evaporation to yield a more concentrated and less inhibitory stream, adapted for bioconversion. Finally, guaiacol was quantitatively converted to muconic acid through bioconversion using an engineered Pseudomonas putida strain containing cytochrome P450 and ferredoxin reductase for guaiacol assimilation and deletion of the native catBC genes for muconic acid production. Isomerization of muconic acid in a fermentation medium depending on pH was also studied.

中文翻译：

---

使用工程恶臭假单胞菌KT2440 和来自硫酸盐木质素的富含愈创木酚的馏分生产粘康酸

工业木质素如牛皮纸木质素是可再生化学品和材料的丰富原料。在这项研究中，开发了一种硫酸盐木质素解聚工艺，随后进行升级分离步骤，并将获得的单芳族化合物进一步生物转化为粘康酸。首先，使用碱催化解聚将工业硫酸盐木质素 Indulin AT 加工成富含愈创木酚的流。该流随后使用液-液萃取和蒸发进行升级，以产生更浓缩且抑制性更低的流，适用于生物转化。最后，使用工程化的恶臭假单胞菌通过生物转化将愈创木酚定量转化为粘康酸含有细胞色素 P450 和铁氧还蛋白还原酶的菌株，用于愈创木酚同化和缺失用于产生粘康酸的天然猫BC 基因。还研究了发酵培养基中粘康酸取决于 pH 值的异构化。