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A Highly Sensitive Label-free Aptasensor Based on Gold Nanourchins and Carbon Nanohorns for the Detection of Lipocalin-2 (LCN-2)
Analytical Sciences ( IF 1.6 ) Pub Date : 2021-06-10 , DOI: 10.2116/analsci.20p303
Chitra Padmakumari KURUP 1 , Noor Faizah MOHD-NAIM 2 , Chaker TLILI 3 , Minhaz Uddin AHMED 1
Affiliation  

A synergistic nanocomposite film composed of gold nanourchins (AuNU), oxidised carbon nanohorns (CNH), and chitosan functioned as an electrode modifier in the fabrication of the sensitive lipocalin-2 (LCN-2) aptasensor. The AuNUs/CNH/CS composite increased the surface area and thereby amplified the signal transduction. The amine-terminated LCN-2 aptamer was immobilised through the amide bond formed between the carboxyl group of polyglutamic acid (PGA) and the amine group of aptamer. Interaction of LCN-2 with the aptamer caused conformational changes in the structure of the aptamer. This generated higher conductivity, resulting in increased DPV peak current. The DPV signal increased with increasing concentration of LCN-2, and the change in signal was used for quantitative detection. The proposed aptasensor was able to detect LCN-2 in the linear range of 0.1 – 100.0 pg mL−1, with a low detection limit of 10 fg mL−1. The aptasensor showed high sensitivity, selectivity, reproducibility, and was able to detect LCN-2 in serum samples.

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中文翻译:

基于金纳米粒和碳纳米角的高灵敏度无标记适体传感器用于检测脂质运载蛋白-2 (LCN-2)

由金纳米海胆 (AuNU)、氧化碳纳米角 (CNH) 和壳聚糖组成的协同纳米复合膜在制备灵敏的 lipocalin-2 (LCN-2) 适体传感器中用作电极改性剂。AuNUs/CNH/CS 复合材料增加了表面积,从而放大了信号转导。胺封端的 LCN-2 适配体通过聚谷氨酸 (PGA) 的羧基和适配体的胺基之间形成的酰胺键固定。LCN-2 与适体的相互作用导致适体结构的构象变化。这会产生更高的电导率,从而导致 DPV 峰值电流增加。DPV信号随着LCN-2浓度的增加而增加,信号的变化用于定量检测。-1,低检测限为 10 fg mL -1。适体传感器显示出高灵敏度、选择性、重现性,并且能够检测血清样品中的 LCN-2。

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更新日期:2021-06-09
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