A detection system consisting of a photothermal heterodyne interferometer (PHI) combined with semi-micro HPLC (high-performance liquid chromatography) has been designed and investigated. An ultraviolet (UV) or deep-UV laser emitting at 375 or 213 nm, respectively, was used for the excitation of nitro-polycyclic aromatic hydrocarbons (NPAHs) and amino acids. A photothermally induced change in the refractive index of the solvent causes an optical phase difference between two arms of the interferometer, one beam passing through the photoexcited region and another used as a reference, which was sensitively detected with the PHI. The separation and detection of NPAHs and amino acids were successfully demonstrated via semi-micro HPLC with the PHI and a UV detector. The detection limits of the UV-excitation PHI for NPAHs were 1.2 – 5.2 times better than that of the commercial UV detector, although the first demonstration of deep-UV excitation suffered from significant baseline fluctuation.

设计并研究了由光热外差干涉仪 (PHI) 与半微量 HPLC（高效液相色谱）相结合的检测系统。分别发射波长为 375 或 213 nm 的紫外 (UV) 或深紫外激光用于激发硝基多环芳烃 (NPAH) 和氨基酸。溶剂折射率的光热引起的变化导致干涉仪两个臂之间的光学相位差，一个光束穿过光激发区域，另一个用作参考，用 PHI 灵敏地检测到。NPAHs和氨基酸的分离和检测成功通过带有 PHI 和 UV 检测器的半微量 HPLC。NPAH 的紫外激发 PHI 检测限比商用紫外检测器的检测限高 1.2 – 5.2 倍，尽管深紫外激发的首次演示遭受了显着的基线波动。