This article reports simple, robust, and species-independent methods for establishing in vitro cell suspension and shoot cultures useful for sustainable bioprospecting of Hypericum, a genus containing several medicinal plants commonly used in treating mild and moderate depression. Loose cell masses or shoot buds were directly induced by culturing seeds on medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) or 6-benzyladenine (BA). Homogeneous cell suspension cultures could be achieved when cells were transferred to liquid culture medium, and robust shoot cultures could be established by transferring cultures containing shoot buds to basal medium. Stable cell suspension and (or) shoot cultures could be established for all Hypericum spp. tested (H. androsaemum L., H. linariifolium Vahl, H. elodes L., H. pulchrum L., H. humifusum L., H. undulatum Schousb. ex Willd, H. perfoliatum L., H. canariense L., H. tomentosum L., H. perforatum L. and H. maculatum Crantz), regardless of their habits and habitats.

本文报告了用于建立体外细胞悬浮液和芽培养物的简单、稳健且不依赖于物种的方法，这些方法可用于金丝桃属植物的可持续生物勘探，金丝桃属植物含有多种常用于治疗轻度和中度抑郁症的药用植物。通过在含有 2,4-二氯苯氧乙酸 (2,4-D) 或 6-苄基腺嘌呤 (BA) 的培养基上培养种子直接诱导松散的细胞团或芽芽。当细胞被转移到液体培养基中时，可以获得均匀的细胞悬浮培养，而通过将含有芽芽的培养物转移到基础培养基中，可以建立健壮的芽培养物。可以为所有金丝桃属植物建立稳定的细胞悬浮液和（或）芽培养物。测试（H. androsaemum L. , H. linariifolium Vahl , H. elodes L. , H. pulchrum L. , H. humifusum L. , H. undulatum Schousb. ex Willd 、H. perfoliatum L. 、H. canariense L. 、H. tomentosum L. 、H. perforatum L. 和H. maculatum Crantz)，无论它们的习性和栖息地如何。