Purpose

Mung bean is a host of the root-lesion nematode Pratylenchus thornei (Sher & Allen) and the beneficial symbionts arbuscular mycorrhizal fungi (AMF) and nitrogen-fixing Bradyrhizobium bacteria. The purpose of this research was to investigate interactions among these organisms affecting their reproduction and functional impact on mung bean nodulation, nutrition, biological nitrogen fixation, growth and seed yield.

Methods

A glasshouse experiment was conducted with mung bean in pots of a pasteurised vertisol using a factorial design of treatments to investigate the interactive effects of AMF, Bradyrhizobium and P. thornei. The plants were assessed at 6 and 12 weeks after sowing for variables of shoot biomass, seed yield, nodulation, P. thornei population density, AMF colonisation of the roots, and nutrients in the plant shoot, including nitrogen isotope natural abundance (δ¹⁵N) to quantify fixed nitrogen.

Results

Arbuscular mycorrhizal fungi and rhizobia acted synergistically to substantially increase nodulation, nitrogen fixation, nutrition, seed yield and biomass of the plants. The population density of P. thornei in roots of the mung bean at 12 weeks increased in plants inoculated with AMF and was positively correlated with plant nutrition namely increased phosphorus, zinc and copper concentrations in the plant shoot.

Conclusion

Understanding these interactions should inform changes in agronomic practices, to promote the synergism between mycorrhiza and rhizobia for mung bean yield, while managing to limit P. thornei population densities to benefit mung bean itself and subsequent crops in the farming system.

中文翻译：

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丛枝菌根真菌与 Bradyrhizobium sp 协同作用。改善绿豆（Vigna radiata）的结瘤、固氮、植物生长和种子产量，但增加了根病线虫 Pratylenchus thornei 的种群密度

目的

绿豆是根病线虫Pratylenchus thornei (Sher & Allen) 和有益共生体丛枝菌根真菌 (AMF) 和固氮慢生根瘤菌的宿主。本研究的目的是调查这些生物之间的相互作用，影响它们的繁殖以及对绿豆结瘤、营养、生物固氮、生长和种子产量的功能影响。

方法

使用因子设计的处理方法，在装有绿豆的巴氏杀菌锅中对绿豆进行温室试验，以研究 AMF、慢生根瘤菌和P. thornei 的交互作用。在播种后第 6 周和第 12 周评估植物的枝条生物量、种子产量、结瘤、P. thornei种群密度、根的 AMF 定植和植物枝条中的营养物质，包括氮同位素天然丰度 (δ ¹⁵ N ) 来量化固定氮。

结果

丛枝菌根真菌和根瘤菌协同作用显着增加植物的结瘤、固氮、营养、种子产量和生物量。在接种 AMF 的植物中，第 12 周绿豆根中桑树的种群密度增加，并且与植物营养呈正相关，即植物芽中磷、锌和铜浓度的增加。

结论

了解这些相互作用应该为农艺实践的变化提供信息，以促进菌根和根瘤菌之间对绿豆产量的协同作用，同时设法限制P. thornei种群密度以使绿豆本身和农业系统中的后续作物受益。