Heat shock protein (HSP101) function as molecular chaperones and confer thermotolerance to plants. In the present investigation, identification, comprehensive expression analysis, phylogeny and protein modelling of HSP101 gene has been done in Aegilops speltoides accession Pau3583. In the present study, we cloned and in silico characterized a HSP101C gene designated as AsHSP101C-Pau3583. AsHSP101C-Pau3583 is 4180 bp long with seven exons and six introns and encoded a polypeptide of 910 amino acids predicted by FGENESH. We have identified 58 SNPs between the AsHSP101C-Pau3583 and reference gene sequence extracted from Ae. speltoides TGAC assembly. Real-time RT-PCR analysis of expression levels of HSP101 gene in two wheat genotypes under heat stress revealed that gene namely HSP101C was up-regulated in Aegilops speltoides acc. Pau3583 by > fourfold in comparison to Triticum aestivum cv. PBW343 under heat stress signifies that it plays a role in conferring heat tolerance. Sequence comparison and phylogenetic analysis of AsHSP101C-Pau3583 with seven wheat homologs Triticum aestivum, Aegilops speltoides (TGAC), Triticum durum cv Cappelli, Triticum durum cv Strongfield, Triticum monococcum, Aegilops tauschii and Triticum urartu showed significant similarities with highly conserved coding regions and functional domains (AAA, AAA + 2, ClpB domains), suggesting the conserved function of HSP101C in different species. The illustration of the protein models of HSP101C in homologs provided information for the ATP-binding motifs within the nucleotide binding domains (NBD), specific for the chaperone activity. These findings are important and identified SNPs could be used for designing markers for ensuring the transfer of AsHSP101C-Pau3583 gene into hexaploid wheat and its role in heat tolerance.

热休克蛋白 (HSP101) 作为分子伴侣发挥作用并赋予植物耐热性。在目前的调查中，已经对Aegilops speltoides Pau3583进行了HSP101基因的鉴定、综合表达分析、系统发育和蛋白质建模。在本研究中，我们克隆并在计算机上表征了一个 HSP101C 基因，命名为AsHSP101C-Pau3583。AsHSP101C-Pau3583长 4180 bp，具有 7 个外显子和 6 个内含子，编码 FGENESH 预测的 910 个氨基酸的多肽。我们已经确定了AsHSP101C-Pau3583和从Ae中提取的参考基因序列之间的 58 个 SNP。魔芋TGAC 组装。热胁迫下两种小麦基因型 HSP101 基因表达水平的实时 RT-PCR 分析表明 HSP101C 基因在Aegilops speltoides acc .中上调。与普通小麦品种相比，Pau3583 增加了 4 倍。热应激下的PBW343表明它在赋予耐热性方面发挥作用。AsHSP101C-Pau3583与七个小麦同源物Triticum aestivum、Aegilops speltoides (TGAC)、Triticum durum cv Cappelli 、Triticum durum cv Strongfield 、Triticum monococcum、Aegilops tauschii和Triticum urartu 的序列比较和系统发育分析与高度保守的编码区和功能域（AAA、AAA + 2、ClpB 域）显示出显着相似性，表明 HSP101C 在不同物种中的保守功能。同源物中 HSP101C 蛋白质模型的图示提供了核苷酸结合域 (NBD) 内的 ATP 结合基序的信息，该基序对伴侣活性具有特异性。这些发现很重要，鉴定的 SNP 可用于设计标记，以确保AsHSP101C-Pau3583基因转移到六倍体小麦及其在耐热性中的作用。