Sogatella furcifera Horvath, commonly known as the white-backed planthoppers (WBPH), is an important pest in East Asian rice fields. Fungal endosymbiosis is widespread among planthoppers in the infraorder Fulgoromorpha and suborder Auchenorrhyncha. We successfully obtained complete mitogenome of five WBPH fungal endosymbionts, belonging to the Ophiocordycipitaceae family, from next-generation sequencing (NGS) reads obtained from S. furcifera samples. These five mitogenomes range in length from 55,390 bp to 55,406 bp, which is shorter than the mitogenome of the fungal endosymbiont found in Ricania speculum, black planthoppers. Twenty-eight protein-coding genes (PCGs), 12 tRNAs, and 2 rRNAs were found in the mitogenomes. Two single-nucleotide polymorphisms, two insertions, and three deletions were identified among the five mitogenomes, which were fewer in number than those of four species of Ophiocordycipitaceae, Ophiocordyceps sinensis, Hirsutella thompsonii, Hirsutella rhossiliensis, and Tolypocladium inflatum. Noticeably short lengths (up to 18 bp) of simple sequence repeats were identified in the five WBPH fungal endosymbiont mitogenomes. Phylogenetic analysis based on conserved PCGs across 25 Ophiocordycipitaceae mitogenomes revealed that the five mitogenomes were clustered with that of R. speculum, forming an independent clade. In addition to providing the full mitogenome sequences, obtaining complete mitogenomes of WBPH endosymbionts can provide insights into their phylogenetic positions without needing to isolate the mtDNA from the host. This advantage is of value to future studies involving fungal endosymbiont mitogenomes.

中文翻译：

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白背飞虱 Sogatella furcifera 真菌内共生体完整线粒体基因组的比较分析

Sogatella furcifera Horvath，俗称白背飞虱（WBPH），是东亚稻田的重要害虫。真菌内共生广泛存在于下目 Fulgoromorpha 和 Auchenorrhyncha 亚目的飞虱中。我们从S. furcifera样本获得的下一代测序 (NGS) 读数中成功获得了属于 Ophiocordycipitaceae 科的五种 WBPH 真菌内共生体的完整有丝分裂基因组。这五个有丝分裂基因组的长度从 55,390 bp 到 55,406 bp 不等，这比在Ricania 窥器中发现的真菌内共生体的有丝分裂基因组要短，黑飞虱。在有丝分裂基因组中发现了 28 个蛋白质编码基因 (PCG)、12 个 tRNA 和 2 个 rRNA。在5个有丝分裂基因组中鉴定出2个单核苷酸多态性、2个插入和3个缺失，数量少于Ophiocordycipitaceae、Ophiocordyceps sinensis、Hirsutella thompsonii、Hirsutella rhossiliensis和Tolypocladium inflatum 4种。在 5 个 WBPH 真菌内共生体有丝分裂基因组中发现了明显较短（最多 18 bp）的简单序列重复序列。基于 25 个 Ophiocordycipitaceae 有丝分裂基因组的保守 PCG 的系统发育分析显示，这五个有丝分裂基因组与R. speculum 的有丝分裂基因组聚类，形成一个独立的进化枝。除了提供完整的丝裂基因组序列外，获得 WBPH 内共生体的完整丝裂基因组可以深入了解它们的系统发育位置，而无需从宿主中分离 mtDNA。这一优势对未来涉及真菌内共生体丝裂基因组的研究具有价值。