当前位置: X-MOL 学术Int. J. Environ. Health Res. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Gene expression in human umbilical vein endothelial cells exposed to fine particulate matter: RNA sequencing analysis
International Journal of Environmental Health Research ( IF 3.2 ) Pub Date : 2021-06-09 , DOI: 10.1080/09603123.2021.1935785
Zhixiang Zhou 1 , Mengnan Qin 1 , Sara Khodahemmati 1, 2 , Wenke Li 1 , Bingyu Niu 1 , Jiangshuai Li 1 , Yanghua Liu 1 , Jingfeng Gao 2
Affiliation  

ABSTRACT

Exposure to airborne particulate matter (PM2.5) is associated with cardiovascular diseases. In order to investigate the molecular mechanisms of air pollution-induced CVDs toxicity, human umbilical vein endothelial cells (HUVECs) were exposed to PM2.5 collected from January, 2016 winter in Beijing, China. We performed RNA sequencing to elucidate key molecular mechanism of PM 2.5-mediated toxicity in HUVECs. A total of 1753 genes, 864 up-regulated and 889 down-regulated, were observed to be differentially expressed genes (DEGs). Among these, genes involved in metabolic response, oxidative stress, inflammatory response, and vascular dysfunction were significantly differentially expressed (log2 FC > 4). The results were validated by quantitative real-time PCR (qPCR) and Western blot for CYP1B1, HMOX1, IL8, and GJA4. Pathway analysis revealed that DEGs were involved in the biological processes related to metabolism, inflammation, and host defense against environmental insults. This research is providing a further understanding of the mechanisms underlying PM2.5-induced cardiovascular diseases (CVDs).

更新日期:2021-06-09
down
wechat
bug