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Mathematical modeling of the process of sterilizing potato explants and obtaining viable potato microclones
IOP Conference Series: Earth and Environmental Science Pub Date : 2021-06-08 , DOI: 10.1088/1755-1315/786/1/012035
N I Lebed 1, 2 , A M Makarov 1 , I V Volkov 1 , M P Kukhtik 1 , M B Lebed 1
Affiliation  

The aim of the studies is obtaining optimal regime parameters of the process of decontamination of plants’ explants by investigating integral action of sterilizing agent and by modifying nutrient medium. The studies of integral influence of technological factors, which have an impact on the process of explants’ sterilization and output of viable plants, have been carried out by the method of experimental design. As a result of the analysis of literature data and searching experiments three main controllable factors, which influence on a percentage of viable potato sprouts, have been highlighted: a concentration of the antibiotic Cefotaxime, a concentration of the cytokinin of the 6-benzylaminopurine (6-BAP) and a concentration of the commercial drug Belizna (the active substance is sodium hypochlorite), as output index – a percentage of viable plants. Rechtschafner design for three-factor experiment has been realized to study optimum region. The optimal values of regime parameters of the process of decontamination of explants of plants’ material have been determined on the basis of regression equations and two-dimensional sections of yield surfaces: the concentration of the antibiotic Cefotaxime in the nutrient medium – 335…365 mg/l, the concentration of the phytohormone 6-BAP - 0.95.1.05 mg/l, the concentration of the commercial drug Belizna (the active substance is sodium hypochlorite) – 2.5.2.6 %. At that the percentage of viable plants will amount to 86.0 %.



中文翻译:

马铃薯外植体灭菌和获得可行马铃薯微克隆过程的数学模型

研究的目的是通过研究灭菌剂的整体作用和通过改变营养培养基来获得植物外植体去污过程的最佳方案参数。采用试验设计的方法,对影响外植体灭菌过程和成活植物产量的工艺因素的综合影响进行了研究。作为对文献数据的分析和搜索实验的结果,三个主要的可控因素影响了可存活马铃薯芽的百分比,已被强调:抗生素头孢噻肟的浓度、6-苄氨基嘌呤的细胞分裂素浓度(6 -BAP)和商业药物Belizna的浓度(活性物质是次氯酸钠),作为产出指数——可存活植物的百分比。实现了三因素试验的Rechtschafner设计来研究最佳区域。根据回归方程和产量表面的二维截面确定了植物材料外植体净化过程的方案参数的最佳值:营养培养基中抗生素头孢噻肟的浓度 – 335…365 mg /l,植物激素 6-BAP 的浓度 - 0.95.1.05 mg/l,商业药物 Belizna 的浓度(活性物质是次氯酸钠) - 2.5.2.6 %。届时,可存活植物的百分比将达到 86.0 %。根据回归方程和产量表面的二维截面确定了植物材料外植体净化过程的方案参数的最佳值:营养培养基中抗生素头孢噻肟的浓度 – 335…365 mg /l,植物激素 6-BAP 的浓度 - 0.95.1.05 mg/l,商业药物 Belizna 的浓度(活性物质是次氯酸钠) - 2.5.2.6 %。届时,可存活植物的百分比将达到 86.0 %。根据回归方程和产量表面的二维截面确定了植物材料外植体净化过程的方案参数的最佳值:营养培养基中抗生素头孢噻肟的浓度 – 335…365 mg /l,植物激素 6-BAP 的浓度 - 0.95.1.05 mg/l,商业药物 Belizna 的浓度(活性物质是次氯酸钠) - 2.5.2.6 %。届时,可存活植物的百分比将达到 86.0 %。商业药物 Belizna 的浓度(活性物质是次氯酸钠) – 2.5.2.6 %。届时,可存活植物的百分比将达到 86.0 %。商业药物 Belizna 的浓度(活性物质是次氯酸钠) – 2.5.2.6 %。届时,可存活植物的百分比将达到 86.0 %。

更新日期:2021-06-08
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