The present study aimed to investigate the capability of a solvent-tolerant bacterium, Bacillus megaterium IBB_Po17, to synthesise silver nanoparticles (AgNPs) through either intracellular or extracellular activities, and to establish if their production was affected when the cells were grown in the presence of organic solvents. The intracellular and extracellular biosynthesis of AgNPs by B. megaterium IBB_Po17 was first confirmed by visual observation and UV–vis spectroscopy and further analysed by high-performance thin-layer chromatography (HPTLC) and transmission electron microscopy (TEM). The antibacterial activity of the synthesised AgNPs was investigated by the agar diffusion method. The intracellular and extracellular biosynthesis of AgNPs by B. megaterium IBB_Po17 when the cells were grown or not in the presence of organic solvents (i.e., n-decane, n-hexadecane, n-hexane, cyclohexane) was confirmed visually by the appearance of a yellowish-brown colour in the reaction mixture or a dark precipitate, as well as by using UV–vis spectroscopy (surface plasmon resonance peaks at 425–455 nm) and TEM analyses (particle with spherical shape, size ranging from 1–85 nm). The biosynthesised AgNPs showed excellent antibacterial activity against several Gram-positive and Gram-negative bacteria. Due to their characteristics, the biosynthesised AgNPs could be used as antibacterial agents, but they could have many other applications.

本研究旨在研究一种耐溶剂细菌巨大芽孢杆菌IBB _Po17通过细胞内或细胞外活动合成银纳米粒子 (AgNPs) 的能力，并确定当细胞在存在的条件下生长时它们的生产是否受到影响有机溶剂。巨大芽孢杆菌IBB _Po17对 AgNPs 的细胞内和细胞外生物合成首先通过目视观察和紫外-可见光谱证实，并通过高效薄层色谱 (HPTLC) 和透射电子显微镜 (TEM) 进一步分析。通过琼脂扩散法研究合成的AgNPs的抗菌活性。通过的AgNPs的胞内和胞外生物合成的巨大芽孢杆菌IBB _Po17当细胞生长或没有有机溶剂（即，存在Ñ -癸烷，Ñ十六烷，Ñ-己烷，环己烷）通过反应混合物中黄褐色或深色沉淀的出现，以及通过使用紫外-可见光谱（表面等离子体共振峰在 425-455 nm）和 TEM 分析（球形颗粒，尺寸范围为 1-85 nm）。生物合成的 AgNPs 对几种革兰氏阳性和革兰氏阴性细菌显示出优异的抗菌活性。由于其特性，生物合成的 AgNPs 可用作抗菌剂，但它们还有许多其他应用。