Recently, there is a growing demand for the polymer-nanoparticle hybrid system due to their long-term stability against aggregation and enhanced functionality for different applications in both material science and biology. In the current study, we have prepared a novel stimuli-responsive biocompatible polymer-silver nanoparticle hybrid using polymeric microgel particles as a micro-reactor in an in-situ treatment, and these hybrid particles are characterized using UV-Vis, dynamic light scattering, and transmission electron microscope with respect to their size distribution and optical properties. Next, these hybrid particles were tested for antibacterial and cytotoxic activities against Salmonella typhimurium bacteria and HeLa cancer cells, respectively using bulk-diffusion, agar diffusion assay, and MTT assay. Both bulk and agar diffusion assays confirm the antibacterial properties of the hybrid microgel-silver nanoparticles against Salmonella typhimurium. Agar diffusion assay shows a systematic trend in the increase of the width of inhibition zone as a function of increasing particle concentration and thus relating the linear relationship between hybrid nanoparticle concentration and concentration of inhibited bacteria. Similarly, linear behavior is also observed with MTT assay, where cell viability decreases linearly with an increase in hybrid particle concentration. Thus, we believe that our polymer-nanoparticle hybrid system may have a wide range of industrial applications as an antibacterial agent and in cancer therapy applications.

最近，由于聚合物-纳米颗粒混合系统具有抗聚集的长期稳定性和在材料科学和生物学的不同应用中增强的功能，因此对聚合物-纳米颗粒混合系统的需求不断增长。在目前的研究中，我们使用聚合物微凝胶颗粒作为原位处理中的微反应器制备了一种新型的刺激响应生物相容性聚合物-银纳米颗粒杂化物，这些杂化物颗粒使用紫外-可见光、动态光散射、和透射电子显微镜的尺寸分布和光学特性。接下来，测试这些混合颗粒对鼠伤寒沙门氏菌的抗菌和细胞毒性活性细菌和 HeLa 癌细胞，分别使用体扩散法、琼脂扩散法和 MTT 法。本体和琼脂扩散试验都证实了混合微凝胶-银纳米颗粒对鼠伤寒沙门氏菌的抗菌特性. 琼脂扩散试验显示抑菌圈宽度随颗粒浓度增加而增加的系统趋势，因此将混合纳米颗粒浓度与抑制细菌浓度之间的线性关系联系起来。类似地，使用 MTT 测定也观察到线性行为，其中细胞活力随着混合颗粒浓度的增加而线性降低。因此，我们相信我们的聚合物-纳米颗粒混合系统作为抗菌剂和癌症治疗应用可能具有广泛的工业应用。