The paper presents the results of studying the interactions of oxidized nanodiamonds with lysozyme in water. It was found that lysozyme is actively adsorbed on the surface of nanodiamonds in two layers. For the first time, using the CARS method, it was found that the conformation of lysozyme molecules in the first adsorption layer changes, and the conformation of molecules adsorbed in the second layer almost coincides with the conformation of free lysozyme molecules. The obtained results and the analysis of the literature data concerning the activity of lysozyme allowed us to conclude that lysozyme in the first adsorption layer mainly loses its enzymatic activity, and in the second—retains it.

本文介绍了研究氧化纳米金刚石与水中溶菌酶相互作用的结果。发现溶菌酶以两层形式活跃地吸附在纳米金刚石表面。首次利用CARS方法发现第一吸附层溶菌酶分子构象发生变化，第二层吸附分子构象与游离溶菌酶分子构象几乎一致。获得的结果和有关溶菌酶活性的文献数据的分析使我们得出结论，第一吸附层中的溶菌酶主要失去其酶活性，而在第二层中保留它。