The development of a multifunctional noncontact manipulation method is desired in cell manipulation. In this paper, we theoretically study and experimentally demonstrate the method of cell manipulation based on swirl. We analyze the swirl flow field features and the cell manipulation mechanism. The swirl is constructed by jetting fluid through three microtubules, and the cells are manipulated using the low pressure of stagnation point and the constant vorticity in the flow field. The experimental results indicate that the cell can be trapped robustly using the stagnation point of swirl. Moreover, the cell rotates with the same angular velocity at any position of the swirl flow field, and the cell is transported along with the movement of the stagnation point. Finally, we extend the application of swirl to manipulate multiple cells. Overall, this method can achieve trapping, rotating, transporting, pairing and enriching for cells, which realizes multifunctional cell manipulation.

在细胞操作中需要开发一种多功能的非接触式操作方法。在本文中，我们从理论上研究和实验证明了基于漩涡的细胞操作方法。我们分析了旋流流场特征和细胞操纵机制。漩涡是通过三个微管喷射流体来构建的，细胞是利用驻点的低压和流场中的恒定涡度来操纵的。实验结果表明，利用涡流的驻点可以很好地捕获细胞。而且，细胞在涡流流场的任何位置都以相同的角速度旋转，细胞随着驻点的运动而被输送。最后，我们扩展了 swirl 的应用来操纵多个细胞。全面的，