Analyses of illicit drugs and medicines in forensic laboratories require constant improvements and updates in protocols and analytical techniques in order to follow the new trends employed by illicit producers. Therefore, applying reliable and fast techniques to perform the chemical profiling of seized samples, allowing for detection of the different types of falsification performed, is an urgent measure. In this study, Paper Spray Ionization (PSI) coupled to Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR MS) was used for determining the chemical profiling of 92 samples of counterfeit medicines and ecstasy tablets. Afterwards, all the samples were evaluated using principal component analysis (PCA) to distinguish their chemical composition. From the results, different strategies for medicine counterfeiting were identified, such as changing the API and employing a mixture of substances to produce distinct medicines. Cross contamination of medicines with cocaine and 3,4-methylenedioxymethamphetamine (MDMA) was also detected. Moreover, the compounds cassaidine and glyceryl nonivamide were identified in all three classes of the seized medicines analyzed. Such compounds act as new markers in illicit production, not reported before in counterfeit medicines. The majority of ecstasy tablets contained MDMA and ethylone in their composition. Using PSI-MS combined with chemometrics, substances present in the seized medicines and ecstasy tablets could be detected through a simple, fast, and reliable procedure. Therefore, it was possible to connect the substances present in the seized material according to their chemical profiling, thus producing important evidence for police intelligence, which constitutes a great tool to be employed in forensic laboratories.

法医实验室对非法药物和药物的分析需要不断改进和更新协议和分析技术，以便跟上非法生产者采用的新趋势。因此，应用可靠和快速的技术对检获的样本进行化学分析，以检测不同类型的伪造行为，是一项紧迫的措施。在本研究中，纸喷雾电离 (PSI) 与傅立叶变换离子回旋共振质谱 (FT-ICR MS) 联用被用于确定 92 份假药和摇头丸样品的化学分析。然后，使用主成分分析（PCA）对所有样品进行评估，以区分它们的化学成分。根据结果​​，确定了不同的药品假冒策略，例如改变 API 和使用混合物质来生产不同的药物。还检测到药物与可卡因和 3,4-亚甲二氧基甲基苯丙胺 (MDMA) 的交叉污染。此外，在所分析的所有三类缉获药物中均鉴定出卡萨西丁和甘油壬酰胺化合物。此类化合物在非法生产中充当新标记，以前在假药中没有报道过。大多数摇头丸的成分中含有摇头丸和乙酮。使用 PSI-MS 结合化学计量学，可以通过简单、快速和可靠的程序检测缉获的药物和摇头丸中存在的物质。因此，可以根据化学分析将被查获材料中存在的物质联系起来，