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Microwave-Assisted Extraction of Ocimum basilicum L. Seed, Trigonella foenum-graecum Seed, and Plantago ovata Forsk Seed Husk Hydrocolloids Compared with Conventional Heating Extraction at Optimum Extraction Conditions
Arabian Journal for Science and Engineering ( IF 2.9 ) Pub Date : 2021-06-08 , DOI: 10.1007/s13369-021-05792-4
Shima Keisandokht , Valérie Orsat , Salwa Karboune , Bhalamurugan Gatamaneni Loganathan

The microwave-assisted extractions (MAE) of Ocimum basilicum L. (basil) seed, Trigonella foenum-graecum (fenugreek) seed, and Plantago ovata Forsk (psyllium) seed husk hydrocolloids (BSH, FSH, and PSH, respectively) were performed at their maximum desirability function (for BSH) or optimum (for FSH and PSH) conditions and compared with conventional heating extraction (CHE). The extraction conditions had been defined based on two responses (Yield % and viscosity, mPa s) after relevant post-screening or optimization experiments based on previous experiments. Higher (17.89, 12.33, 32.60, 30.19, 71.73%) average extraction yields were obtained for BSH extracted by MAE (MAE-BSH), BSH extracted by CHE (CHE-BSH), FSH extracted by MAE (MAE-FSH), FSH extracted by CHE (CHE-FSH), and PSH extracted by MAE (MAE-PSH) in respective order compared with the estimated values (15.03, 9.96, 32, 22, and 65.4). Molecular weight (Mw) measurements were executed and the average Mw was determined to be 3.93 × 106, 2.89 × 106, 6.81 × 106 Da in the heavier fractions of BSH, FSH, and PSH. The FT-IR spectroscopy was performed and the predominant absorbance bands were defined. Dielectric properties of the 2% BSH, FSH, and PSH solutions were found to be comparable with distilled water. The steady flow analysis showed shear-thinning behavior for BSH, FSH, and PSH. The apparent viscosity (η, mPa s) of PSH was the highest followed by BSH and FSH. Dynamic flow measurements showed characteristics of a weak gel for BSH and PSH. Scanning electron microscopy showed morphological variations between the studied hydrocolloids. In addition, the study also indicated that microwave heating has lower destructive effects on the functional properties of the extract as compared to conventional thermal processing and could pave the way for future research. To conclude, MAE was found to be the efficient method for extraction of hydrocolloids with higher yield at a shorter extraction time.



中文翻译:

微波辅助提取罗勒种子、胡芦巴种子和车前子 Forsk 种子壳水胶体与常规加热提取在最佳提取条件下的比较

Ocimum basilicum L.(罗勒)种子、Trigonella foenum-graecum(葫芦巴)种子和车前子的微波辅助提取 (MAE)Forsk(车前草)种子壳水胶体(分别为 BSH、FSH 和 PSH)在其最大合意函数(对于 BSH)或最佳(对于 FSH 和 PSH)条件下进行,并与传统加热提取 (CHE) 进行比较。在基于先前实验的相关后筛选或优化实验之后,基于两个响应(产率%和粘度,mPa·s)定义了提取条件。MAE(MAE-BSH)提取的BSH、CHE(CHE-BSH)提取的BSH、MAE(MAE-FSH)提取的FSH、FSH的平均提取率更高(17.89、12.33、32.60、30.19、71.73%) CHE (CHE-FSH) 和 MAE (MAE-PSH) 提取的 PSH 分别与估计值 (15.03、9.96、32、22 和 65.4) 进行比较。进行分子量 (Mw) 测量,平均 Mw 确定为 3.93 × 106 , 2.89 × 10 6 , 6.81 × 10 6  Da 在 BSH、FSH 和 PSH 的较重馏分中。进行了 FT-IR 光谱并确定了主要的吸收带。发现 2% BSH、FSH 和 PSH 溶液的介电特性与蒸馏水相当。稳态流动分析显示了 BSH、FSH 和 PSH 的剪切稀化行为。表观粘度 ( η, mPa s) 的 PSH 最高,其次是 BSH 和 FSH。动态流量测量显示 BSH 和 PSH 的弱凝胶特性。扫描电子显微镜显示所研究的水胶体之间的形态变化。此外,该研究还表明,与传统热处理相比,微波加热对提取物功能特性的破坏性影响较小,可为未来的研究铺平道路。总而言之,MAE 被发现是在更短的提取时间内以更高的产量提取水胶体的有效方法。

更新日期:2021-06-08
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