ABSTRACT

Paeonia delavayi is listed as an endangered species in the southwest of China. The flower colours of cultivated examples of this species are most commonly yellow and red, but a much wider range of flower colours can be found in plants growing in the natural habitat. To elucidate the basis of the colour range, pigments were extracted from flower petals of seven selected colours and profiled and quantified using High Performance Liquid Chromatography (HPLC) and Liquid Chromatography–Mass Spectrometry (LC-MS). All the petal colours were found to share the same base pigment types as the yellow flower – a composite colour based on a mix of flavonoids, carotenoids and chlorophyll. Anthocyanin profiling revealed the same anthocyanin compositions in all red coloured flowers, namely, cyanidin-3,5-diglucoside, cyanidin-3-glucoside, cyanidin-3-arabinoside, peonidin-3,5-diglucoside, peonidin-3-glucoside and peonidin 3-arabinoside. Quantitative analysis suggested the yellow colour was principally formed by the flavonoid naringenin chalcone 2'-glucoside (2’,4’,6’,4-tetrahydroxychalcone 2'-glucoside). The various red/orange flower colours were the result of superimposing different amounts of anthocyanins onto the yellow flower background, with carotenoids and chlorophylls having only a minor contribution to the final colour. The results provide useful information for breeding programmes for ornamental varieties of P. delavayi.

摘要

芍药被列为我国西南濒危物种。该物种的栽培实例的花色最常见的是黄色和红色，但在自然栖息地生长的植物中可以找到更广泛的花色。为了阐明颜色范围的基础，从七种选定颜色的花瓣中提取色素，并使用高效液相色谱 (HPLC) 和液相色谱-质谱 (LC-MS) 进行分析和定量。发现所有花瓣颜色与黄花具有相同的基础色素类型——一种基于黄酮类、类胡萝卜素和叶绿素混合物的复合颜色。花青素分析显示所有红色花朵中的花青素成分相同，即 cyanidin-3,5-diglucoside、cyanidin-3-glucoside、cyanidin-3-arabinoside、peonidin-3、5-二葡萄糖苷、牡丹苷-3-葡萄糖苷和牡丹苷3-阿拉伯糖苷。定量分析表明黄色主要由黄酮柚皮素查尔酮2'-葡萄糖苷（2',4',6',4-四羟基查耳酮2'-葡萄糖苷）形成。各种红色/橙色花朵颜色是在黄色花朵背景上叠加不同数量的花青素的结果，类胡萝卜素和叶绿素对最终颜色的贡献很小。研究结果为观赏品种选育提供了有用的信息。各种红色/橙色花朵颜色是在黄色花朵背景上叠加不同数量的花青素的结果，类胡萝卜素和叶绿素对最终颜色的贡献很小。研究结果为观赏品种选育提供了有用的信息。各种红色/橙色花朵颜色是在黄色花朵背景上叠加不同数量的花青素的结果，类胡萝卜素和叶绿素对最终颜色的贡献很小。研究结果为观赏品种选育提供了有用的信息。P.德拉瓦伊。