Abstract

Synergistic antibacterial effect of green synthesized silver nanoparticles combined with antibiotic on Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive) was conducted, respectively. Well diffusion, growth curve, anti-biofilm, and colony formation were applied to evaluate its antimicrobial activity. Significant synergistic effect was found when silver nanoparticles were combined with streptomycin sulfate at ratios of 8:2 and 9:1 for E. coli, while 5:5 and 8:2 for S. aureus. It suggested that silver nanoparticles were proved to be an efficient antibacterial agent, and the challenge like drug resistance would also be able to resolve under the condition of silver nanoparticles combined with antibiotics.

摘要

分别进行了绿色合成银纳米粒子联合抗生素对大肠杆菌（革兰氏阴性）和金黄色葡萄球菌（革兰氏阳性）的协同抗菌作用。应用孔扩散、生长曲线、抗生物膜和菌落形成来评估其抗菌活性。当银纳米颗粒与硫酸链霉素以 8:2 和 9:1 的比例组合时，发现了显着的协同效应，对大肠杆菌，而对金黄色葡萄球菌的比例为5:5 和 8:2 。这表明银纳米粒子被证明是一种有效的抗菌剂，在银纳米粒子与抗生素结合的情况下，耐药性等挑战也将得到解决。