Abstract

A very quick and easy LC-MS/MS analysis method for 5-HIAA (5-hydoxyindoleacetic acid) has been developed. The method was fully validated and proved to work well in a clinical setting. Precision at the upper reference limit 123 nmol/L was 3,3% CV. Accuracy ranged from 96% at low levels (50–100 nmol/L) to 99.7% at high levels (500 nmol/L). A previously reported reference interval of 35–123 nmol/L was confirmed as valid based on analysis of 40 samples from voluntary blood donors.

中文翻译：

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血清中 5-羟基吲哚乙酸 (5-HIAA) 的超快 LC-MS/MS 分析

摘要

已开发出一种非常快速简便的 5-HIAA（5-羟基吲哚乙酸）LC-MS/MS 分析方法。该方法经过充分验证，并证明在临床环境中运行良好。参考上限 123 nmol/L 的精密度为 3.3% CV。准确度范围从低浓度 (50–100 nmol/L) 的 96% 到高浓度 (500 nmol/L) 的 99.7%。根据对来自自愿献血者的 40 个样本的分析，确认了先前报告的 35-123 nmol/L 参考区间是有效的。