Abstract

Researchers have used mixed microbial cultures to degrade various pollutants such as domestic effluents, pharmaceuticals, hydrocarbon molecules, etc. The use of a mixed microbial culture induces synergistic associations between different microorganisms by producing multiple enzymes that can effectively degrade refractory molecules such as azo and anthraquinone dyes. It is in this context that we have been interested in the biological decolorization of contaminated water with an anthraquinone dye Remazol Blue Brilliant R (RBBR), by using microorganisms from activated sludge taken at the outlet of the aeration basin of the wastewater treatment plant from Reghaia (Algeria). In order to stimulate the development of the microorganisms responsible for the secretion of ligninolytic enzymes, we have introduced date pedicels cut into small pieces into the bioreactors. These will serve as co-substrate and support for the microorganisms present in the reaction medium. The parametric study is performed by varying the concentration of activated sludge, the concentration of date pedicels, the pH, the temperature and the initial concentration of the Remazol Blue Brilliant R (RBBR) dye. A maximum elimination yield of 82% is obtained. For an activated sludge/pedicel ratio of dates equals 2 at a neutral pH, T = 30°C and an initial dye concentration of 100 mg/L. We also note that this promising technique has achieved a yield of 53% for an initial concentration of Remazol Blue Brilliant R of 3500 mg/L.

中文翻译：

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使用枣花梗作为生物刺激剂的 Remazol Blue Brilliant R 染料的生物降解

摘要

研究人员使用混合微生物培养物降解各种污染物，如生活污水、药物、碳氢化合物分子等。使用混合微生物培养物通过产生多种酶来诱导不同微生物之间的协同关联，这些酶可以有效降解偶氮和蒽醌等难降解分子染料。正是在这种情况下，我们一直对使用蒽醌染料 Remazol Blue Brilliant R (RBBR) 对污染水进行生物脱色感兴趣，方法是使用来自 Reghaia 废水处理厂曝气池出口处的活性污泥中的微生物（阿尔及利亚）。为了刺激负责木质素分解酶分泌的微生物的发育，我们已将切成小块的枣梗引入生物反应器。这些将作为反应介质中存在的微生物的共底物和支持物。参数研究是通过改变活性污泥浓度、枣梗浓度、pH 值、温度和 Remazol Blue Brilliant R (RBBR) 染料的初始浓度来进行的。获得了 82% 的最大消除率。对于中性 pH 值下的活性污泥/枣梗比等于 2，获得了 82% 的最大消除率。对于中性 pH 值下的活性污泥/枣梗比等于 2，获得了 82% 的最大消除率。对于中性 pH 值下的活性污泥/枣梗比等于 2，T = 30°C，初始染料浓度为 100 mg/L。我们还注意到，对于 3500 mg/L 的 Remazol Blue Brilliant R 初始浓度，这种有前途的技术已实现了 53% 的产率。