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Genetic Polymorphism of 24 Autosomal STR in the Population of Rwanda
Biochemical Genetics ( IF 2.4 ) Pub Date : 2021-06-07 , DOI: 10.1007/s10528-021-10067-6
Gasana Paul 1, 2, 3 , JunLin Liu 1 , Pan Ma 1 , Abiy Wendifraw Assefa 1, 2 , Jingsi Yang 1 , Shaoqing Li 1 , DongKe Fu 2 , Yan Liu 4 , Rutayisire Gad 3 , Kamuhangire Edward 5 , Shengbin Li 1 , Bao Zhang 1 , Chunxia Yan 1
Affiliation  

Rwanda is one of the smallest countries of Africa, where forensic genetic studies are rarely being conducted and very few DNA databases have been developed. Short tandem repeats (STRs) polymorphisms were investigated in 505 unrelated Rwandese by using the HUMDNA TYPING (Yanhuang) Kit. The following STRs were targeted: D3S1358, D13S317, D7S820, D16S539, SE33, D10S1248, D5S818, D21S11, TPOX, D1S1656, D6S1043, D19S433, D22S1045, D8S1179, Penta E, D2S441, D12S391, D2S1338, vWA, Penta D, TH01, D18S51, CSF1PO and FGA. The purpose of this study was to elucidate the genetic diversity and explore the potential of applying these 24 STR in 505 Rwandan population in forensics. A total of 360 alleles, with corresponding allele frequencies in the range from 0.001 to 0.442, were found in the Rwandan population. SE33 presented the highest polymorphism (PIC=0.921) among these 24 loci, whereas D13S317 presented the lowest one (PIC=0.671). No deviation from the Hardy-Weinberg equilibrium was observed for any of the 24 loci. The forensic parameters, including the combined power of discrimination (PD and the combined exclusion power, have demonstrated that this panel of 24 STRs is highly informative and useful for forensic applications such as individuals’ identification and paternity tests. Additionally, the genetic distances between Rwanda population and other 24 published populations were calculated based on 8 overlapping loci with the polygenetic tree revealing significant clusters in the populations associated with their geographic locations and their historical relationship.



中文翻译:

卢旺达人群中24个常染色体STR的遗传多态性

卢旺达是非洲最小的国家之一,很少进行法医遗传学研究,也很少开发 DNA 数据库。使用 HUMDNA TYPING (Yanhuang) Kit 在 505 个不相关的卢旺达人中研究了短串联重复 (STR) 多态性。靶向以下STRS:D3S1358,D13S317,D7S820,​​D16S539,SE33,D10S1248,D5S818,D21S11,TPOX,D1S1656,D6S1043,D19S433,D22S1045,D8S1179,PENTA E,D2S441,D12S391,D2S1338,VWA,Penta D,Th01, D18S51、CSF1PO 和 FGA。本研究的目的是阐明遗传多样性并探索将这 24 个 STR 应用于 505 名卢旺达人口的法医研究的潜力。在卢旺达人群中发现了总共 360 个等位基因,相应的等位基因频率在 0.001 到 0.442 的范围内。SE33 呈现出最高的多态性 (PIC=0. 921)在这 24 个基因座中,而 D13S317 呈现最低的一个(PIC = 0.671)。对于 24 个基因座中的任何一个,都没有观察到与 Hardy-Weinberg 平衡的偏差。法医参数,包括综合歧视能力(PD 和综合排除能力)表明,这组 24 个 STR 具有高度信息性,可用于法医应用,例如个人识别和亲子鉴定。此外,卢旺达之间的遗传距离人口和其他 24 个已发表的人口是基于 8 个重叠基因座计算的,多基因树揭示了与其地理位置及其历史关系相关的人口中的重要集群。对于 24 个基因座中的任何一个,都没有观察到与 Hardy-Weinberg 平衡的偏差。法医参数,包括综合歧视能力(PD 和综合排除能力)表明,这组 24 个 STR 具有高度信息性,可用于法医应用,例如个人识别和亲子鉴定。此外,卢旺达之间的遗传距离人口和其他 24 个已发表的人口是基于 8 个重叠基因座计算的,多基因树揭示了与其地理位置及其历史关系相关的人口中的重要集群。对于 24 个基因座中的任何一个,都没有观察到与 Hardy-Weinberg 平衡的偏差。法医参数,包括综合歧视能力(PD 和综合排除能力)表明,这组 24 个 STR 具有高度信息性,可用于法医应用,例如个人识别和亲子鉴定。此外,卢旺达之间的遗传距离人口和其他 24 个已发表的人口是基于 8 个重叠基因座计算的,多基因树揭示了与其地理位置及其历史关系相关的人口中的重要集群。已经证明,这个由 24 个 STR 组成的小组对于法医应用(例如个人识别和亲子鉴定)具有高度的信息性和有用性。此外,卢旺达人口与其他 24 个已发表人口之间的遗传距离是基于 8 个重叠基因座计算的,多基因树揭示了与其地理位置及其历史关系相关的人口中的重要集群。已经证明,这个由 24 个 STR 组成的小组对于法医应用(例如个人识别和亲子鉴定)具有高度的信息性和有用性。此外,卢旺达人口与其他 24 个已发表人口之间的遗传距离是基于 8 个重叠基因座计算的,多基因树揭示了与其地理位置及其历史关系相关的人口中的重要集群。

更新日期:2021-06-08
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