A biosensor for phosphate determination with the flow-injection system was developed using rhodium nanoparticles modified Poly(pyrrole-co-[1-(2-aminophenyl) pyrrole])/pyruvate oxidase. The biosensor showed a very wide linearity up to 70 mM phosphate concentration compared to previously reports, response time of 4 s., operational stability with a relative standard deviation of 0.009 % and accuracy of 99.4 %±0.949 at a flow rate of 2.0 Ml min.⁻¹ at exactly −0.68 V. Detection limit were calculated to be 21±0.001 μM by preserving 81.1 % of its initial response at the end of 16^th days. Artificial urine was analyzed without dilution to investigate biosensor performance.

中文翻译：

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新型流动注射铑纳米粒子修饰磷酸盐生物传感器及其在人工尿液中的操作

使用铑纳米颗粒修饰的聚（吡咯-co- [1-（2-氨基苯基）吡咯]）/丙酮酸氧化酶，开发了一种通过流动注射系统测定磷酸盐的生物传感器。与之前的报告相比，该生物传感器在高达 70 mM 磷酸盐浓度时显示出非常宽的线性，响应时间为 4 秒，操作稳定性，相对标准偏差为 0.009 %，准确度为 99.4 %±0.949，流速为 2.0 毫升/分钟. ^-1正好-0.68 V.检测下限分别计算为21±0.001μM通过保持在16结束它的初始响应的81.1％^第天。未经稀释分析人造尿液以研究生物传感器性能。