The influence of excessive metal–organic frameworks (MOFs) on the translation process was investigated in this study. Different concentrations and sizes of MOFs were tested through adding them to a cell-free expression system and observing the downstream expression signal. Furthermore, a proteomics analysis has been employed to explore and reveal the influence of MOFs on the protein expression, which can provide significant referential value for developing a delivery system with MOFs.

中文翻译：

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金属-有机框架对蛋白质表达的影响

本研究调查了过量金属有机骨架（MOF）对翻译过程的影响。通过将它们添加到无细胞表达系统并观察下游表达信号来测试不同浓度和大小的 MOF。此外，蛋白质组学分析已被用于探索和揭示 MOFs 对蛋白质表达的影响，这可以为开发具有 MOFs 的传递系统提供重要的参考价值。